Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis
Carvalho, Claudia M.B.
Altheimer, Alyssa N.
Lupski, James R.
Brennand, Kristen J.Note: Order does not necessarily reflect citation order of authors.
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CitationTCW, J., C. M. Carvalho, B. Yuan, S. Gu, A. N. Altheimer, S. McCarthy, D. Malhotra, et al. 2017. “Divergent Levels of Marker Chromosomes in an hiPSC-Based Model of Psychosis.” Stem Cell Reports 8 (3): 519-528. doi:10.1016/j.stemcr.2017.01.010. http://dx.doi.org/10.1016/j.stemcr.2017.01.010.
AbstractSummary In the process of generating presumably clonal human induced pluripotent stem cells (hiPSCs) from two carriers of a complex structural rearrangement, each having a psychotic disorder, we also serendipitously generated isogenic non-carrier control hiPSCs, finding that the rearrangement occurs as an extrachromosomal marker (mar) element. All confirmed carrier hiPSCs and differentiated neural progenitor cell lines were found to be mosaic. We caution that mar elements may be difficult to functionally evaluate in hiPSC cultures using currently available methods, as it is difficult to distinguish cells with and without mar elements in live mosaic cultures.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:32071982
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