Show simple item record

dc.contributor.authorLu, Ming-Yingen_US
dc.contributor.authorHuang, Ching-Ien_US
dc.contributor.authorHsieh, Ming-Yenen_US
dc.contributor.authorHsieh, Tusty-Juanen_US
dc.contributor.authorHsi, Edwarden_US
dc.contributor.authorTsai, Pei-Chienen_US
dc.contributor.authorTsai, Yi-Shanen_US
dc.contributor.authorLin, Ching-Chihen_US
dc.contributor.authorHsieh, Meng-Hsuanen_US
dc.contributor.authorLiang, Po-Chengen_US
dc.contributor.authorLin, Yi-Hungen_US
dc.contributor.authorHou, Nai-Jenen_US
dc.contributor.authorYeh, Ming-Lunen_US
dc.contributor.authorHuang, Chung-Fengen_US
dc.contributor.authorLin, Zu-Yauen_US
dc.contributor.authorChen, Shinn-Cherngen_US
dc.contributor.authorHuang, Jee-Fuen_US
dc.contributor.authorChuang, Wan-Longen_US
dc.contributor.authorDai, Chia-Yenen_US
dc.contributor.authorYu, Ming-Lungen_US
dc.date.accessioned2017-04-06T03:19:19Z
dc.date.issued2016en_US
dc.identifier.citationLu, M., C. Huang, M. Hsieh, T. Hsieh, E. Hsi, P. Tsai, Y. Tsai, et al. 2016. “Dynamics of PBMC gene expression in hepatitis C virus genotype 1-infected patients during combined peginterferon/ribavirin therapy.” Oncotarget 7 (38): 61325-61335. doi:10.18632/oncotarget.11348. http://dx.doi.org/10.18632/oncotarget.11348.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:32072065
dc.description.abstractHepatitis C virus (HCV) can replicate in peripheral blood mononuclear cells (PBMCs), which can produce interferon to defend against virus infection. We hypothesized that dynamic gene expression in PBMCs might impact the treatment efficacy of peginterferon/ribavirin in HCV patients. PBMCs were collected at baseline, 1st week and 4th week of treatment from 27 chronic HCV-1 patients with 48-week peginterferon/ribavirin therapy (screening dataset n = 7; validation dataset n = 20). A sustained virologic response (SVR) was defined as undetectable HCV RNA throughout the 24 weeks after end-of-treatment. A complete early virologic response (cEVR) was defined as negative HCV RNA at treatment week 12. Forty-three differentially expressed genes identified by Affymetrix microarray were validated by quantitative polymerase chain reaction. Thirteen genes at week 1 and 24 genes at week 4 were upregulated in the SVR group compared with the non-SVR group. We selected 8 target genes (RSAD2, LOC26010, HERC5, HERC6, IFI44, SERPING1, IFITM3, and DDX60) at week 1 as the major components of the predictive model. This predictive model reliably stratified the responders and non-responders at week 1 (AUC = 0.89, p = 0.007 for SVR; AUC = 0.95, p = 0.003 for cEVR), especially among patients carrying the IL28B rs8099917 TT genotype (AUC = 0.89, p = 0.02 for SVR; AUC = 1.0, p = 0.008 for cEVR). The performance of this predictive model was superior to traditional predictors, including the rapid virologic response, viral load and IL28B genotype.en
dc.language.isoen_USen
dc.publisherImpact Journals LLCen
dc.relation.isversionofdoi:10.18632/oncotarget.11348en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5308654/pdf/en
dash.licenseLAAen_US
dc.subjecthepatitis Cen
dc.subjectinterferonen
dc.subjectsustained virologic responseen
dc.titleDynamics of PBMC gene expression in hepatitis C virus genotype 1-infected patients during combined peginterferon/ribavirin therapyen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalOncotargeten
dash.depositing.authorYu, Ming-Lungen_US
dc.date.available2017-04-06T03:19:19Z
dc.identifier.doi10.18632/oncotarget.11348*
dash.authorsorderedfalse
dash.contributor.affiliatedYu, Ming-Lung


Files in this item

Thumbnail

This item appears in the following Collection(s)

Show simple item record