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dc.contributor.authorHenry, Whitney S.en_US
dc.contributor.authorHendrickson, David G.en_US
dc.contributor.authorBeca, Franciscoen_US
dc.contributor.authorGlass, Benjaminen_US
dc.contributor.authorLindahl-Allen, Marianneen_US
dc.contributor.authorHe, Lizhien_US
dc.contributor.authorJi, Zheen_US
dc.contributor.authorStruhl, Kevinen_US
dc.contributor.authorBeck, Andrew H.en_US
dc.contributor.authorRinn, John L.en_US
dc.contributor.authorToker, Alexen_US
dc.date.accessioned2017-04-06T03:19:45Z
dc.date.issued2016en_US
dc.identifier.citationHenry, W. S., D. G. Hendrickson, F. Beca, B. Glass, M. Lindahl-Allen, L. He, Z. Ji, et al. 2016. “LINC00520 is induced by Src, STAT3, and PI3K and plays a functional role in breast cancer.” Oncotarget 7 (50): 81981-81994. doi:10.18632/oncotarget.11962. http://dx.doi.org/10.18632/oncotarget.11962.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:32072115
dc.description.abstractLong non-coding RNAs (lncRNAs) have been implicated in normal cellular homeostasis as well as pathophysiological conditions, including cancer. Here we performed global gene expression profiling of mammary epithelial cells transformed by oncogenic v-Src, and identified a large subset of uncharacterized lncRNAs potentially involved in breast cancer development. Specifically, our analysis revealed a novel lncRNA, LINC00520 that is upregulated upon ectopic expression of oncogenic v-Src, in a manner that is dependent on the transcription factor STAT3. Similarly, LINC00520 is also increased in mammary epithelial cells transformed by oncogenic PI3K and its expression is decreased upon knockdown of mutant PIK3CA. Additional expression profiling highlight that LINC00520 is elevated in a subset of human breast carcinomas, with preferential enrichment in the basal-like molecular subtype. ShRNA-mediated depletion of LINC00520 results in decreased cell migration and loss of invasive structures in 3D. RNA sequencing analysis uncovers several genes that are differentially expressed upon ectopic expression of LINC00520, a significant subset of which are also induced in v-Src-transformed MCF10A cells. Together, these findings characterize LINC00520 as a lncRNA that is regulated by oncogenic Src, PIK3CA and STAT3, and which may contribute to the molecular etiology of breast cancer.en
dc.language.isoen_USen
dc.publisherImpact Journals LLCen
dc.relation.isversionofdoi:10.18632/oncotarget.11962en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5347668/pdf/en
dash.licenseLAAen_US
dc.subjectlncRNAen
dc.subjectSrcen
dc.subjectPI3Ken
dc.subjectbreast canceren
dc.subjectLINC00520en
dc.titleLINC00520 is induced by Src, STAT3, and PI3K and plays a functional role in breast canceren
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalOncotargeten
dash.depositing.authorBeca, Franciscoen_US
dc.date.available2017-04-06T03:19:45Z
dc.identifier.doi10.18632/oncotarget.11962*
dash.authorsorderedfalse
dash.contributor.affiliatedBeca, Francisco
dash.contributor.affiliatedJi, Zhe
dash.contributor.affiliatedStruhl, Kevin
dash.contributor.affiliatedHe, Lizhi
dash.contributor.affiliatedToker, Alex
dash.contributor.affiliatedBeck, Andrew
dash.contributor.affiliatedRinn, John


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