dc.contributor.author | Ouyang, Mengxing | en_US |
dc.contributor.author | Hill, Winfield | en_US |
dc.contributor.author | Lee, Jung Hyun | en_US |
dc.contributor.author | Hur, Soojung Claire | en_US |
dc.date.accessioned | 2017-04-06T03:21:03Z | |
dc.date.issued | 2017 | en_US |
dc.identifier.citation | Ouyang, Mengxing, Winfield Hill, Jung Hyun Lee, and Soojung Claire Hur. 2017. “Microscale Symmetrical Electroporator Array as a Versatile Molecular Delivery System.” Scientific Reports 7 (1): 44757. doi:10.1038/srep44757. http://dx.doi.org/10.1038/srep44757. | en |
dc.identifier.issn | | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:32072166 | |
dc.description.abstract | Successful developments of new therapeutic strategies often rely on the ability to deliver exogenous molecules into cytosol. We have developed a versatile on-chip vortex-assisted electroporation system, engineered to conduct sequential intracellular delivery of multiple molecules into various cell types at low voltage in a dosage-controlled manner. Micro-patterned planar electrodes permit substantial reduction in operational voltages and seamless integration with an existing microfluidic technology. Equipped with real-time process visualization functionality, the system enables on-chip optimization of electroporation parameters for cells with varying properties. Moreover, the system’s dosage control and multi-molecular delivery capabilities facilitate intracellular delivery of various molecules as a single agent or in combination and its utility in biological research has been demonstrated by conducting RNA interference assays. We envision the system to be a powerful tool, aiding a wide range of applications, requiring single-cell level co-administrations of multiple molecules with controlled dosages. | en |
dc.language.iso | en_US | en |
dc.publisher | Nature Publishing Group | en |
dc.relation.isversionof | doi:10.1038/srep44757 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5357946/pdf/ | en |
dash.license | LAA | en_US |
dc.title | Microscale Symmetrical Electroporator Array as a Versatile Molecular Delivery System | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | Scientific Reports | en |
dash.depositing.author | Hill, Winfield | en_US |
dc.date.available | 2017-04-06T03:21:03Z | |
dc.identifier.doi | 10.1038/srep44757 | * |
dash.contributor.affiliated | Hill, I. | |