How the glycosyltransferase OGT catalyzes amide bond cleavage
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CitationJanetzko, John, Sunia A. Trauger, Michael B. Lazarus, and Suzanne Walker. 2016. “How the glycosyltransferase OGT catalyzes amide bond cleavage.” Nature chemical biology 12 (11): 899-901. doi:10.1038/nchembio.2173. http://dx.doi.org/10.1038/nchembio.2173.
AbstractThe essential human enzyme O-GlcNAc transferase (OGT), known for modulating the functions of nuclear and cytoplasmic proteins through Ser/Thr glycosylation, was unexpectedly implicated in the proteolytic maturation of the cell cycle regulator host cell factor-1 (HCF-1). Here we show that HCF-1 cleavage occurs via glycosylation of a glutamate side chain followed by on-enzyme formation of an internal pyroglutamate, which undergoes spontaneous backbone hydrolysis.
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