Transcriptional dynamics of endodermal organ formation
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Sherwood, Richard I.
Chen, Tzong-Yang Albert
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CitationSherwood, Richard I., Tzong-Yang Albert Chen, and Douglas A. Melton. 2009. Transcriptional dynamics of endodermal organ formation. Developmental Dynamics 238(1): 29-42.
AbstractAlthough endodermal organs including the liver, pancreas, and intestine are of signiﬁcant therapeutic interest, the mechanism by which the endoderm is divided into organ domains during embryogenesis is not well understood. To better understand this process, global gene expression proﬁling was performed on early endodermal organ domains. This global analysis was followed up by dynamic immunoﬂuorescence analysis of key transcription factors, uncovering novel expression patterns as well as cell surface proteins that allow prospective isolation of speciﬁc endodermal organ domains. Additionally, a repressive interaction between Cdx2 and Sox2 was found to occur at the prospective stomach–intestine border, with the hepatic and pancreatic domains forming at this boundary, and Hlxb9 was revealed to have graded expression along the dorsal–ventral axis. These results contribute to understanding the mechanism of endodermal organogenesis and should assist efforts to replicate this process using pluripotent stem cells.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:32116879
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