Identification of Angiogenesis/Metastases Genes Predicting Chemoradiotherapy Response in Patients With Laryngopharyngeal Carcinoma

DSpace/Manakin Repository

Identification of Angiogenesis/Metastases Genes Predicting Chemoradiotherapy Response in Patients With Laryngopharyngeal Carcinoma

Citable link to this page

 

 
Title: Identification of Angiogenesis/Metastases Genes Predicting Chemoradiotherapy Response in Patients With Laryngopharyngeal Carcinoma
Author: Ganly, Ian; Talbot, Simon George; Carlson, Diane; Viale, Agnes; Maghami, Ellie; Osman, Iman; Sherman, Eric; Pfister, David; Chuai, Shaokun; Shaha, Ashok R.; Kraus, Dennis; Shah, Jatin P.; Socci, Nicholas D.; Singh, Bhuvanesh

Note: Order does not necessarily reflect citation order of authors.

Citation: Ganly, Ian, Simon Talbot, Diane Carlson, Agnes Viale, Ellie Maghami, Iman Osman, Eric Sherman, et al. 2007. “Identification of Angiogenesis/Metastases Genes Predicting Chemoradiotherapy Response in Patients With Laryngopharyngeal Carcinoma.” Journal of Clinical Oncology 25 (11) (April 10): 1369–1376. doi:10.1200/jco.2005.05.3397.
Access Status: Full text of the requested work is not available in DASH at this time (“dark deposit”). For more information on dark deposits, see our FAQ.
Full Text & Related Files:
Abstract: Purpose
To identify genes related to angiogenesis/metastasis that predict locoregional failure in patients with laryngopharyngeal cancer (LPC) undergoing chemoradiotherapy (CRT) treatment.
Methods
Tumor tissue was collected and snap-frozen from 35 sequential patients with histologically confirmed LPC being treated with CRT. Gene expression analysis was performed using a novel cDNA array consisting of 277 genes functionally associated with angiogenesis (n 152) and/or metastasis (n 125). Locoregional response was correlated to the gene expression profiles to identify genes associated with outcome. These genes were internally validated by real-time reverse transcriptase polymerase chain reaction (RT-PCR) and validated externally by immunohis- tochemistry analysis on an independent set of patients.
Results
Locoregional failure occurred in nine of 35 patients. Seventeen genes from the cDNA microarray correlated with locoregional failure (two-sample t test, P .05). Seven genes were chosen for additional analysis based on the availability of antibodies for immunohistochemistry. Of these seven genes, real-time RT-PCR validated four genes: MDM2, VCAM-1, erbB2, and H-ras (Wilcoxon rank sum test, P .008, .02, .04, and .04, respectively). External validation by immunohistochem- istry confirmed MDM2 and erbB2 as being predictive of locoregional response. Controlling for stage of disease, positivity for MDM2 or erbB2 was an independent negative predictor of locoregional disease-free survival.
Conclusion
Genomic screening by cDNA microarray and validation internally by real-time RT-PCR and externally by immunohistochemistry have identified two genes (MDM2 and erbB2) as predictors of locoregional failure in LPC patients treated with CRT. The role of these genes in treatment selection and the functional basis for their activity in CRT response merit additional consideration.
Published Version: 10.1200/jco.2005.05.3397
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:32416075
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters