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dc.contributor.authorUmeda, Hideyuki
dc.contributor.authorAikawa, Masanori
dc.contributor.authorLibby, Peter
dc.date.accessioned2017-04-27T20:05:25Z
dc.date.issued2011
dc.identifier.citationUmeda, Hideyuki, Masanori Aikawa, and Peter Libby. 2011. “Liberation of Desmosine and Isodesmosine as Amino Acids from Insoluble Elastin by Elastolytic Proteases.” Biochemical and Biophysical Research Communications 411, no. 2: 281–286.en_US
dc.identifier.issn0006-291Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:32605695
dc.description.abstractThe development of atherosclerotic lesions and abdominal aortic aneurysms involves degradation and loss of extracellular matrix components, such as collagen and elastin. Releases of the elastin cross-links desmosine (DES) and isodesmosine (IDE) may reflect elastin degradation in cardiovascular diseases. This study investigated the production of soluble elastin cross-linking structures by proteinases implicated in arterial diseases. Recombinant MMP-12 and neutrophil elastase liberated DES and IDE as amino acids from insoluble elastin. DES and IDE were also released from insoluble elastin exposed to monocyte/macrophage cell lines or human primary macrophages derived from peripheral blood monocytes. Elastin oxidized by reactive oxygen species (ROS) liberated more unconjugated DES and IDE than did non-oxidized elastin when incubated with MMP-12 or neutrophil elastase. These results support the exploration of free DES and IDE as biomarkers of elastin degradation.en_US
dc.language.isoen_USen_US
dc.publisherElsevier BVen_US
dc.relation.isversionofdoi:10.1016/j.bbrc.2011.06.124en_US
dc.relation.hasversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3148299/en_US
dash.licenseLAA
dc.subjectAtherosclerosisen_US
dc.subjectBiomarkeren_US
dc.subjectCross-linken_US
dc.subjectDesmosineen_US
dc.subjectElastin degradationen_US
dc.subjectIsodesmosineen_US
dc.titleLiberation of desmosine and isodesmosine as amino acids from insoluble elastin by elastolytic proteasesen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalBiochemical and Biophysical Research Communicationsen_US
dash.depositing.authorLibby, Peter
dc.date.available2017-04-27T20:05:25Z
dc.identifier.doi10.1016/j.bbrc.2011.06.124*
dash.contributor.affiliatedAikawa, Masanori
dash.contributor.affiliatedLibby, Peter


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