Gene expression analysis uncovers novel hedgehog interacting protein (HHIP) effects in human bronchial epithelial cells
View/ Open
Author
Sathirapongsasuti, J. Fah
Mancini, John D.
Thibault, Derek M.
Bakke, Per S.
Gulsvik, Amund
Lomas, David A.
Beaty, Terri H.
Geigenmuller, Ute
Rennard, Stephen I.
Choi, Augustine M.K.
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1016/j.ygeno.2013.02.010Metadata
Show full item recordCitation
Zhou, Xiaobo, Weiliang Qiu, J. Fah Sathirapongsasuti, Michael H. Cho, John D. Mancini, Taotao Lao, Derek M. Thibault, et al. 2013. Gene Expression Analysis Uncovers Novel Hedgehog Interacting Protein (HHIP) Effects in Human Bronchial Epithelial Cells. Genomics 101, no. 5: 263–272. doi:10.1016/j.ygeno.2013.02.010.Abstract
Hedgehog Interacting Protein (HHIP) was implicated in chronic obstructive pulmonary disease (COPD) by genome-wide association studies (GWAS). However, it remains unclear how HHIP contributes to COPD pathogenesis. To identify genes regulated by HHIP, we performed gene expression microarray analysis in a human bronchial epithelial cell line (Beas-2B) stably infected with HHIP shRNAs. HHIP silencing led to differential expression of 296 genes; enrichment for variants nominally associated with COPD was found. Eighteen of the differentially expressed genes were validated by real-time PCR in Beas-2B cells. Seven of 11 validated genes tested in human COPD and control lung tissues demonstrated significant gene expression differences. Functional annotation indicated enrichment for extracellular matrix and cell growth genes. Network modeling demonstrated that the extracellular matrix and cell proliferation genes influenced by HHIP tended to be interconnected. Thus, we identified potential HHIP targets in human bronchial epithelial cells that may contribute to COPD pathogenesis.Terms of Use
This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAACitable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:32684125
Collections
- HMS Scholarly Articles [17917]
Contact administrator regarding this item (to report mistakes or request changes)