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dc.contributor.authorZhou, Xiaobo
dc.contributor.authorQiu, Weiliang
dc.contributor.authorSathirapongsasuti, J. Fah
dc.contributor.authorCho, Michael Hyosang
dc.contributor.authorMancini, John D.
dc.contributor.authorLao, Taotao
dc.contributor.authorThibault, Derek M.
dc.contributor.authorLitonjua, Augusto Ampil
dc.contributor.authorBakke, Per S.
dc.contributor.authorGulsvik, Amund
dc.contributor.authorLomas, David A.
dc.contributor.authorBeaty, Terri H.
dc.contributor.authorHersh, Craig Palmer
dc.contributor.authorAnderson, Christopher David
dc.contributor.authorGeigenmuller, Ute
dc.contributor.authorRaby, Benjamin Alexander
dc.contributor.authorRennard, Stephen I.
dc.contributor.authorPerrella, Mark A.
dc.contributor.authorChoi, Augustine M.K.
dc.contributor.authorQuackenbush, John
dc.contributor.authorSilverman, Edwin Kepner
dc.date.accessioned2017-05-15T20:02:28Z
dc.date.issued2013
dc.identifier.citationZhou, Xiaobo, Weiliang Qiu, J. Fah Sathirapongsasuti, Michael H. Cho, John D. Mancini, Taotao Lao, Derek M. Thibault, et al. 2013. Gene Expression Analysis Uncovers Novel Hedgehog Interacting Protein (HHIP) Effects in Human Bronchial Epithelial Cells. Genomics 101, no. 5: 263–272. doi:10.1016/j.ygeno.2013.02.010.en_US
dc.identifier.issn0888-7543en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:32684125
dc.description.abstractHedgehog Interacting Protein (HHIP) was implicated in chronic obstructive pulmonary disease (COPD) by genome-wide association studies (GWAS). However, it remains unclear how HHIP contributes to COPD pathogenesis. To identify genes regulated by HHIP, we performed gene expression microarray analysis in a human bronchial epithelial cell line (Beas-2B) stably infected with HHIP shRNAs. HHIP silencing led to differential expression of 296 genes; enrichment for variants nominally associated with COPD was found. Eighteen of the differentially expressed genes were validated by real-time PCR in Beas-2B cells. Seven of 11 validated genes tested in human COPD and control lung tissues demonstrated significant gene expression differences. Functional annotation indicated enrichment for extracellular matrix and cell growth genes. Network modeling demonstrated that the extracellular matrix and cell proliferation genes influenced by HHIP tended to be interconnected. Thus, we identified potential HHIP targets in human bronchial epithelial cells that may contribute to COPD pathogenesis.en_US
dc.language.isoen_USen_US
dc.publisherElsevier BVen_US
dc.relation.isversionofdoi:10.1016/j.ygeno.2013.02.010en_US
dash.licenseLAA
dc.subjectHedgehog interacting protein (HHIP)en_US
dc.subjectgene expression profilingen_US
dc.subjectCOPD (Chronic obstructive pulmonary disease)en_US
dc.subjectextracellular matrix (ECM)en_US
dc.subjectnetwork modelingen_US
dc.titleGene expression analysis uncovers novel hedgehog interacting protein (HHIP) effects in human bronchial epithelial cellsen_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalGenomicsen_US
dash.depositing.authorZhou, Xiaobo
dc.date.available2017-05-15T20:02:28Z
dc.identifier.doi10.1016/j.ygeno.2013.02.010*
dash.authorsorderedfalse
dash.contributor.affiliatedQiu, Weiliang
dash.contributor.affiliatedZhou, Xiaobo
dash.contributor.affiliatedLao, Taotao
dash.contributor.affiliatedHersh, Craig
dash.contributor.affiliatedAnderson, Christopher
dash.contributor.affiliatedLitonjua, Augusto A.
dash.contributor.affiliatedRaby, Benjamin
dash.contributor.affiliatedPerrella, Mark
dash.contributor.affiliatedQuackenbush, John
dash.contributor.affiliatedSilverman, Edwin
dash.contributor.affiliatedCho, Michael
dc.identifier.orcid0000-0002-2702-5879


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