De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds

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De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds

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Title: De novo assembly of the Aedes aegypti genome using Hi-C yields chromosome-length scaffolds
Author: Dudchenko, Olga; Batra, Sanjit S.; Omer, Arina D.; Nyquist, Sarah K.; Hoeger, Marie; Durand, Neva C.; Shamim, Muhammad S.; Machol, Ido; Lander, Eric Steven; Aiden, Aviva ORCID  0000-0002-5445-0969 ; Aiden, Erez Lieberman

Note: Order does not necessarily reflect citation order of authors.

Citation: Dudchenko,Olga, Sanjit S. Batra, Arina D. Omer, Sarah K. Nyquist, Marie Hoeger, Neva C. Durand, Muhammad S. Shamim, et al. 2017. “De Novo Assembly of the Aedes Aegypti Genome Using Hi-C Yields Chromosome-Length Scaffolds .” Science (March 23): eaal3327. doi:10.1126/science.aal3327
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Abstract: The Zika outbreak, spread by the Aedes aegypti mosquito, highlights the need to
create high-quality assemblies of large genomes in a rapid and cost-effective fashion. Here, we
combine Hi-C data with existing draft assemblies to generate chromosome-length scaffolds. We
validate this method by assembling a human genome, de novo, from short reads alone (67X
coverage). We then combine our method with draft sequences to create genome assemblies of
the mosquito disease vectors Aedes aegypti and Culex quinquefasciatus, each consisting of three
scaffolds corresponding to the three chromosomes in each species. These assemblies indicate
that virtually all genomic rearrangements among these species occur within, rather than between,
chromosome arms. The genome assembly procedure we describe is fast, inexpensive, accurate,
and can be applied to many species.
Published Version: doi:10.1126/science.aal3327
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:32689443
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