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dc.contributor.authorMaxwell, M
dc.contributor.authorNaber, S P
dc.contributor.authorWolfe, H J
dc.contributor.authorGalanopoulos, T
dc.contributor.authorHedley-Whyte, E. Tessa
dc.contributor.authorBlack, Peter McLaren
dc.contributor.authorAntoniades, H N
dc.date.accessioned2017-05-17T16:57:34Z
dc.date.issued1990
dc.identifierQuick submit: 2017-03-22T11:30:33-0400
dc.identifier.citationMaxwell, M, S P Naber, H J Wolfe, T Galanopoulos, E T Hedley-Whyte, P M Black, and H N Antoniades. 1990. “Coexpression of Platelet-Derived Growth Factor (PDGF) and PDGF-Receptor Genes by Primary Human Astrocytomas May Contribute to Their Development and Maintenance.” Journal of Clinical Investigation 86 (1) (July 1): 131–140. doi:10.1172/jci114675.en_US
dc.identifier.issn0021-9738en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:32697829
dc.description.abstractThe present studies investigated the expression of the two PDGF genes (c-sis/PDGF-2 and PDGF-1) and the PDGF-receptor b gene (PDGF-R) in 34 primary human astrocytomas. Northern blot analysis demonstrated the coexpression of the c-sis/PDGF-2 protooncogene and the PDGF-R gene in all astrocytomas examined. The majority of the tumors also expressed the PDGF-1 gene. There was no correlation between the expression of the two PDGF genes. Nonmalignant human brain tissue expressed the PDGF-R and PDGF-1 genes but not the c-sis/PDGF-2 protooncogene. In situ hybridization of astrocytoma tissue localized the expression of the c-sis and PDGF-R mRNA's in tumor cells. Capillary endothelial cells also expressed c-sis mRNA. In contrast, nonmalignant human brain tissue expressed only PDGF-R mRNA but not c-sis/PDGF-2 mRNA. The coexpression of a potent mitogenic growth factor protooncogene (c-sis) and its receptor gene in astrocytoma tumor cells suggests the presence of an autocrine mechanism that may contribute to the development and maintenance of astrocytomas. The expression of c-sis mRNA in tumor cells but not in nonmalignant brain cells may serve as an additional diagnostic criterion for the detection of astrocytomas in small tissue specimen using in situ hybridization for the detection of c-sis mRNA and/or immunostaining for the recognition of its protein product.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Society for Clinical Investigationen_US
dc.relation.isversionof10.1172/jci114675en_US
dash.licenseLAA
dc.subjectplatelet-derived growth factoren_US
dc.subjectc-sis protooncogeneen_US
dc.subjectPDGF-recepten_US
dc.subjectPDGF and PDGF-receptor genesen_US
dc.subjectautocrine functionen_US
dc.subjectin situ hybridizationen_US
dc.titleCoexpression of platelet-derived growth factor (PDGF) and PDGF-receptor genes by primary human astrocytomas may contribute to their development and maintenance.en_US
dc.typeJournal Articleen_US
dc.date.updated2017-03-22T15:30:37Z
dc.description.versionVersion of Recorden_US
dc.relation.journalJournal of Clinical Investigationen_US
dash.depositing.authorHedley-Whyte, E. Tessa
dc.date.available1990
dc.date.available2017-05-17T16:57:34Z
dc.identifier.doi10.1172/jci114675*
dash.contributor.affiliatedMaxwell, Michele M.
dash.contributor.affiliatedBlack, Peter
dash.contributor.affiliatedHedley-Whyte, E.


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