Simultaneous mapping of active DNA demethylation and sister chromatid exchange in single cells

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Simultaneous mapping of active DNA demethylation and sister chromatid exchange in single cells

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Title: Simultaneous mapping of active DNA demethylation and sister chromatid exchange in single cells
Author: Wu, Xiaoji; Inoue, Azusa; Suzuki, Tsukasa; Zhang, Yi

Note: Order does not necessarily reflect citation order of authors.

Citation: Wu, Xiaoji, Azusa Inoue, Tsukasa Suzuki, and Yi Zhang. 2017. “Simultaneous Mapping of Active DNA Demethylation and Sister Chromatid Exchange in Single Cells.” Genes & Development 31 (5) (March 1): 511–523. doi:10.1101/gad.294843.116.
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Abstract: To understand mammalian active DNA demethylation, various methods have been developed to map the genomic distribution of the demethylation intermediates 5-formylcysotine (5fC) and 5-carboxylcytosine (5caC). However, the majority of these methods requires a large number of cells to begin with. In this study, we describe low-input methylase-assisted bisulfite sequencing (liMAB-seq ) and single-cell MAB-seq (scMAB-seq), capable of profiling 5fC and 5caC at genome scale using ∼100 cells and single cells, respectively. liMAB-seq analysis of preimplantation embryos reveals the oxidation of 5mC to 5fC/5caC and the positive correlation between chromatin accessibility and processivity of ten-eleven translocation (TET) enzymes. scMAB-seq captures the cell-to-cell heterogeneity of 5fC and 5caC and reveals the strand-biased distribution of 5fC and 5caC. scMAB-seq also allows the simultaneous high-resolution mapping of sister chromatid exchange (SCE), facilitating the study of this type of genomic rearrangement. Therefore, our study not only establishes new methods for the genomic mapping of active DNA demethylation using limited numbers of cells or single cells but also demonstrates the utilities of the methods in different biological contexts.
Published Version: doi:10.1101/gad.294843.116
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:32784876
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