Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1
Di Costanzo, Stefania
Brown, Robert H
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CitationKiskinis, Evangelos, Jackson Sandoe, Luis A. Williams, Gabriella L. Boulting, Rob Moccia, Brian J. Wainger, Steve Han, et al. 2014. “Pathways Disrupted in Human ALS Motor Neurons Identified through Genetic Correction of Mutant SOD1.” Cell Stem Cell 14 (6) (June): 781–795. doi:10.1016/j.stem.2014.03.004.
AbstractDirect electrical recording and stimulation of neural activity using micro-fabricated silicon and metal micro-wire probes have contributed extensively to basic neuroscience and therapeutic applications; however, the dimensional and mechanical mismatch of these probes with the brain tissue limits their stability in chronic implants and decreases the neuron–device contact. Here, we demonstrate the realization of a three-dimensional macroporous nanoelectronic brain probe that combines ultra-flexibility and subcellular feature sizes to overcome these limitations. Built-in strains controlling the local geometry of the macroporous devices are designed to optimize the neuron/probe interface and to promote integration with the brain tissue while introducing minimal mechanical perturbation. The ultra-flexible probes were implanted frozen into rodent brains and used to record multiplexed local field potentials and single-unit action potentials from the somatosensory cortex. Significantly, histology analysis revealed filling-in of neural tissue through the macroporous network and attractive neuron–probe interactions, consistent with long-term biocompatibility of the device.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:33010381
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