Interaction of Retinoic Acid and scl Controls Primitive Blood Development
View/ Open
1624704.pdf (516.6Kb)
Access Status
Full text of the requested work is not available in DASH at this time ("dark deposit"). For more information on dark deposits, see our FAQ.Author
de Jong, Jill L. O.
Wang, Yuan
Palis, James
Opara, Praise
Pugach, Emily
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1182/blood-2009-10-249557Metadata
Show full item recordCitation
De Jong, Jill L. O., Alan J. Davidson, Yuan Wang, James Palis, Praise Opara, Emily Pugach, George Q. Daley, and Leonard I. Zon. 2010. Interaction of Retinoic Acid and scl Controls Primitive Blood Development. Blood 116, no. 2: 201–209.Abstract
Hematopoietic development during embryogenesis involves the interaction of extrinsic signaling pathways coupled to an intrinsic cell fate that is regulated by cell-specific transcription factors. Retinoic acid (RA) has been linked to stem cell self-renewal in adults and also participates in yolk sac blood island formation. Here, we demonstrate that RA decreases gata1 expression and blocks primitive hematopoiesis in zebrafish (Danio rerio) embryos, while increasing expression of the vascular marker, fli1. Treatment with an inhibitor of RA biosynthesis or a retinoic acid receptor antagonist increases \(gata1^+\) erythroid progenitors in the posterior mesoderm of wild-type embryos and anemic \(cdx4^{−/−}\) mutants, indicating a link between the cdx-hox signaling pathway and RA. Overexpression of scl, a DNA binding protein necessary for hematopoietic development, rescues the block of hematopoiesis induced by RA. We show that these effects of RA and RA pathway inhibitors are conserved during primitive hematopoiesis in murine yolk sac explant cultures and embryonic stem cell assays. Taken together, these data indicate that RA inhibits the commitment of mesodermal cells to hematopoietic fates, functioning downstream of cdx4 and upstream of scl. Our studies establish a new connection between RA and scl during development that may participate in stem cell self-renewal and hematopoietic differentiation.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910607/Citable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:33010418
Collections
- FAS Scholarly Articles [17582]
Contact administrator regarding this item (to report mistakes or request changes)
Comments made during the workflow steps
FAR 2011 Zon emailed 2016-04-22 MM Zon emailed 2017-02-20 MM meta.dark