Interaction of Retinoic Acid and scl Controls Primitive Blood Development

DSpace/Manakin Repository

Interaction of Retinoic Acid and scl Controls Primitive Blood Development

Citable link to this page

 

 
Title: Interaction of Retinoic Acid and scl Controls Primitive Blood Development
Author: de Jong, Jill L. O.; Davidson, Alan; Wang, Yuan; Palis, James; Opara, Praise; Pugach, Emily; Daley, George Quentin; Zon, Leonard Ira

Note: Order does not necessarily reflect citation order of authors.

Citation: De Jong, Jill L. O., Alan J. Davidson, Yuan Wang, James Palis, Praise Opara, Emily Pugach, George Q. Daley, and Leonard I. Zon. 2010. Interaction of Retinoic Acid and scl Controls Primitive Blood Development. Blood 116, no. 2: 201–209.
Access Status: Full text of the requested work is not available in DASH at this time (“dark deposit”). For more information on dark deposits, see our FAQ.
Full Text & Related Files:
Abstract: Hematopoietic development during embryogenesis involves the interaction of extrinsic signaling pathways coupled to an intrinsic cell fate that is regulated by cell-specific transcription factors. Retinoic acid (RA) has been linked to stem cell self-renewal in adults and also participates in yolk sac blood island formation. Here, we demonstrate that RA decreases gata1 expression and blocks primitive hematopoiesis in zebrafish (Danio rerio) embryos, while increasing expression of the vascular marker, fli1. Treatment with an inhibitor of RA biosynthesis or a retinoic acid receptor antagonist increases \(gata1^+\) erythroid progenitors in the posterior mesoderm of wild-type embryos and anemic \(cdx4^{−/−}\) mutants, indicating a link between the cdx-hox signaling pathway and RA. Overexpression of scl, a DNA binding protein necessary for hematopoietic development, rescues the block of hematopoiesis induced by RA. We show that these effects of RA and RA pathway inhibitors are conserved during primitive hematopoiesis in murine yolk sac explant cultures and embryonic stem cell assays. Taken together, these data indicate that RA inhibits the commitment of mesodermal cells to hematopoietic fates, functioning downstream of cdx4 and upstream of scl. Our studies establish a new connection between RA and scl during development that may participate in stem cell self-renewal and hematopoietic differentiation.
Published Version: doi:10.1182/blood-2009-10-249557
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2910607/
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:33010418
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters