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dc.contributor.authorde Jong, J. L. O.
dc.contributor.authorBurns, Caroline Erter
dc.contributor.authorChen, A. T.
dc.contributor.authorPugach, E.
dc.contributor.authorMayhall, E. A.
dc.contributor.authorSmith, A. C. H.
dc.contributor.authorFeldman, Henry Arthur
dc.contributor.authorZhou, Yi
dc.contributor.authorZon, Leonard Ira
dc.date.accessioned2017-06-12T19:42:13Z
dc.date.issued2011
dc.identifier.citationDe Jong, J. L. O., C. E. Burns, A. T. Chen, E. Pugach, E. A. Mayhall, A. C. H. Smith, H. A. Feldman, Y. Zhou, and L. I. Zon. 2011. “Characterization of Immune-Matched Hematopoietic Transplantation in Zebrafish.” Blood 117, no. 16: 4234–4242.en_US
dc.identifier.issn0006-4971en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:33010421
dc.description.abstractEvaluating hematopoietic stem cell (HSC) function in vivo requires a long-term transplantation assay. Although zebrafish are a powerful model for discovering the genetics of hematopoiesis, hematopoietic transplantation approaches have been underdeveloped. Here we established a long-term reconstitution assay in adult zebrafish. Primary and secondary recipients showed multilineage engraftment at 3 months after transplantation. Limiting dilution data suggest that at least 1 in 65 000 zebrafish marrow cells contain repopulating activity, consistent with mammalian HSC frequencies. We defined zebrafish haplotypes at the proposed major histocompatibility complex locus on chromosome 19 and tested functional significance through hematopoietic transplantation. Matching donors and recipients dramatically increased engraftment and percentage donor chimerism compared with unmatched fish. These data constitute the first functional test of zebrafish histocompatibility genes, enabling the development of matched hematopoietic transplantations. This lays the foundation for competitive transplantation experiments with mutant zebrafish HSCs and chemicals to test for effects on engraftment, thereby providing a model for human hematopoietic diseases and treatments not previously available.en_US
dc.description.sponsorshipStem Cell and Regenerative Biologyen_US
dc.language.isoen_USen_US
dc.publisherAmerican Society of Hematologyen_US
dc.relation.isversionofdoi:10.1182/blood-2010-09-307488en_US
dash.licenseMETA_ONLY
dc.titleCharacterization of immune-matched hematopoietic transplantation in zebrafishen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalBlooden_US
dash.depositing.authorZon, Leonard Ira
dash.embargo.until10000-01-01
dc.identifier.doi10.1182/blood-2010-09-307488*
workflow.legacycommentsFAR 2014 Zon emailed 2016-04-22 MM Zon emailed 2017-02-20 MM meta.darken_US
dash.authorsorderedfalse
dash.contributor.affiliatedFeldman, Henry
dash.contributor.affiliatedBurns, Caroline
dash.contributor.affiliatedZhou, Yi
dash.contributor.affiliatedZon, Leonard


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