An aptamer-functionalized chemomechanically modulated biomolecule catch-and-release system

Author
Liu, Ya
Harris, Valerie
Nan, Hanqing
Mujica, Maritza
Vasquez, Yolanda
Bhattacharya, Amitabh
Ma, Yongting
Kuksenok, Olga
Balazs, Anna C.
He, Ximin
Note: Order does not necessarily reflect citation order of authors.
Published Version
https://doi.org/10.1038/NCHEM.2203Metadata
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Shastri, Ankita, Lynn M. McGregor, Ya Liu, Valerie Harris, Hanqing Nan, Maritza Mujica, Yolanda Vasquez, et al. 2015. An Aptamer-Functionalized Chemomechanically Modulated Biomolecule Catch-and-Release System. Nature Chem 7, no. 5: 447–454. doi:10.1038/nchem.2203.Abstract
The efficient extraction of (bio)molecules from fluid mixtures is vital for applications ranging from target characterization in (bio)chemistry to environmental analysis and biomedical diagnostics. Inspired by biological processes that seamlessly synchronize the capture, transport and release of biomolecules, we designed a robust chemo-mechanical sorting system capable of the concerted “catch and release” of target biomolecules from a solution mixture. The hybrid system is composed of target-specific, reversible binding sites attached to microscopic fins embedded in a responsive hydrogel that moves the cargo between two chemically-distinct environments. To demonstrate the utility of the system, we focus on the effective separation of thrombin by synchronizing the pH-dependent binding strength of a thrombin-specific aptamer with volume changes of the pH-responsive hydrogel in a biphasic microfluidic regime, and show the non-destructive separation with quantitative sorting efficiency, system’s stability and amenability to multiple solution recycling.Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:33204049
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