Multicolor Brainbow Imaging in Zebrafish

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Multicolor Brainbow Imaging in Zebrafish

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Title: Multicolor Brainbow Imaging in Zebrafish
Author: Pan, Y. A.; Livet, J.; Sanes, Joshua R.; Lichtman, Jeff; Schier, Alexander F

Note: Order does not necessarily reflect citation order of authors.

Citation: Pan, Y. A., J. Livet, J. R. Sanes, J. W. Lichtman, and A. F. Schier. 2011. “Multicolor Brainbow Imaging in Zebrafish.” Cold Spring Harbor Protocols 2011 (1) (January 1): pdb.prot5546–pdb.prot5546. doi:10.1101/pdb.prot5546.
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Abstract: This protocol describes how to use the Brainbow strategy to label neurons in many different hues. The Brainbow system uses a random Cre/lox recombination to create varied combinations of red, blue, and green fluorescent proteins in each cell. The differences in color allow users to follow multiple cells, regardless of how closely they are positioned. This protocol describes how to use Brainbow imaging in zebrafish and provides examples of how to use color as a guide to trace axonal processes. We use the zebrafish trigeminal sensory ganglion as an example and discuss potential modifications for the general use of this technique.
Published Version: doi:10.1101/pdb.prot5546
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:33431734
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