Multicolor Brainbow Imaging in Zebrafish
Citation
Pan, Y. A., J. Livet, J. R. Sanes, J. W. Lichtman, and A. F. Schier. 2011. “Multicolor Brainbow Imaging in Zebrafish.” Cold Spring Harbor Protocols 2011 (1) (January 1): pdb.prot5546–pdb.prot5546. doi:10.1101/pdb.prot5546.Abstract
This protocol describes how to use the Brainbow strategy to label neurons in many different hues. The Brainbow system uses a random Cre/lox recombination to create varied combinations of red, blue, and green fluorescent proteins in each cell. The differences in color allow users to follow multiple cells, regardless of how closely they are positioned. This protocol describes how to use Brainbow imaging in zebrafish and provides examples of how to use color as a guide to trace axonal processes. We use the zebrafish trigeminal sensory ganglion as an example and discuss potential modifications for the general use of this technique.Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:33431734
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FAR 2012 Lichtman emailed 2016-05-18 MM Lichtman emailed 2017-03-09 MM meta.dark manuscript found on PMC