A Common Origin for B-1a and B-2 Lymphocytes in Clonal Pre- Hematopoietic Stem Cells
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Author
Hadland, Brandon K.
Varnum-Finney, Barbara
Poulos, Michael G.
Butler, Jason M.
Rafii, Shahin
Yoder, Mervin C.
Yoshimoto, Momoko
Bernstein, Irwin D.
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https://doi.org/10.1016/j.stemcr.2017.04.007Metadata
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Hadland, Brandon K., Barbara Varnum-Finney, Pankaj K. Mandal, Derrick J. Rossi, Michael G. Poulos, Jason M. Butler, Shahin Rafii, Mervin C. Yoder, Momoko Yoshimoto, and Irwin D. Bernstein. 2017. “A Common Origin for B-1a and B-2 Lymphocytes in Clonal Pre- Hematopoietic Stem Cells.” Stem Cell Reports 8 (6): 1563-1572. doi:10.1016/j.stemcr.2017.04.007. http://dx.doi.org/10.1016/j.stemcr.2017.04.007.Abstract
Summary Recent evidence points to the embryonic emergence of some tissue-resident innate immune cells, such as B-1a lymphocytes, prior to and independently of hematopoietic stem cells (HSCs). However, whether the full hematopoietic repertoire of embryonic HSCs initially includes these unique lineages of innate immune cells has been difficult to assess due to lack of clonal assays that identify and assess HSC precursor (pre-HSC) potential. Here, by combining index sorting of single embryonic hemogenic precursors with in vitro HSC maturation and transplantation assays, we analyze emerging pre-HSCs at the single-cell level, revealing their unique stage-specific properties and clonal lineage potential. Remarkably, clonal pre-HSCs detected between E9.5 and E11.5 contribute to the complete B cell repertoire, including B-1a lymphocytes, revealing a previously unappreciated common precursor for all B cell lineages at the pre-HSC stage and a second embryonic origin for B-1a lymphocytes.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5469918/pdf/Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:33439360
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