The Cremeomycin Biosynthetic Gene Cluster Encodes a Pathway for Diazo Formation

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The Cremeomycin Biosynthetic Gene Cluster Encodes a Pathway for Diazo Formation

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Title: The Cremeomycin Biosynthetic Gene Cluster Encodes a Pathway for Diazo Formation
Author: Waldman, Abraham Joseph; Pechersky, Yakov; Wang, Peng; Wang, Jennifer X.; Balskus, Emily Patricia

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Citation: Waldman, Abraham J., Yakov Pechersky, Peng Wang, Jennifer X. Wang, and Emily P. Balskus. 2015. “The Cremeomycin Biosynthetic Gene Cluster Encodes a Pathway for Diazo Formation.” ChemBioChem 16 (15) (September 14): 2172–2175. doi:10.1002/cbic.201500407.
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Abstract: Diazo groups are found in a range of natural products that possess potent biological activities. Despite longstanding interest in these metabolites, diazo group biosynthesis is not well understood, in part because of difficulties in identifying specific genes linked to diazo formation. Here we describe the discovery of the gene cluster that produces the o-diazoquinone natural product cremeomycin and its heterologous expression in Streptomyces lividans. We have used stable isotope feeding experiments and in vitro characterization of biosynthetic enzymes to decipher the order of events in this pathway and establish that diazo construction involves latestage N–N bond formation. This work represents the first successful production of a diazocontaining metabolite in a heterologous host, experimentally linking a set of genes with diazo formation.
Published Version: 10.1002/cbic.201500407
Other Sources: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4996270/
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:33445939
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