All-Atom Model for Stabilization of α-Helical Structure in Peptides by Hydrocarbon Staples
MetadataShow full item record
CitationKutchukian, Peter S., Jae Shick Yang, Gregory L. Verdine, and Eugene I. Shakhnovich. 2009. “All-Atom Model for Stabilization of α-Helical Structure in Peptides by Hydrocarbon Staples.” Journal of the American Chemical Society 131 (13) (April 8): 4622–4627. doi:10.1021/ja805037p. http://dx.doi.org/10.1021/ja805037p.
AbstractRecent work has shown that the incorporation of an all-hydrocarbon “staple” into peptides can greatly increase their α-helix propensity, leading to an improvement in pharmaceutical properties such as proteolytic stability, receptor affinity and cell-permeability. Stapled peptides thus show promise as a new class of drugs capable of accessing intractable targets such as those that engage in intracellular protein-protein interactions. The extent of α-helix stabilization provided by stapling has proven to be substantially context dependent, requiring cumbersome screening to identify the optimal site for staple incorporation. In certain cases, a staple encompassing one turn of the helix (attached at residues i and i+4) furnishes greater helix stabilization than one encompassing two turns (i,i+7 staple), which runs counter to expectation based on polymer theory. These findings highlight the need for a more thorough understanding of the forces that underlie helix stabilization by hydrocarbon staples. Here we report all-atom Monte Carlo folding simulations comparing unmodified peptides derived from RNAse A and BID BH3 with various i,i+4 and i,i+7 stapled versions thereof. The results of these simulations were found to be in quantitative agreement with experimentally determined helix propensities. We also discovered that staples can stabilize quasi-stable decoy conformations, and that the removal of these states plays a major role in determining the helix stability of stapled peptides. Finally, we critically investigate why our method works, exposing the underlying physical forces that stabilize stapled peptides.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:33464143
- FAS Scholarly Articles