Analytical Devices Based on Direct Synthesis of DNA on Paper

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Analytical Devices Based on Direct Synthesis of DNA on Paper

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Title: Analytical Devices Based on Direct Synthesis of DNA on Paper
Author: Glavan, Ana C; Niu, Jia; Chen, Zhen; Güder, Firat; Cheng, Chao-Min; Liu, David Ruchien; Whitesides, George McClelland

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Citation: Glavan, Ana C., Jia Niu, Zhen Chen, Firat Güder, Chao-Min Cheng, David Liu, and George M. Whitesides. 2016. “Analytical Devices Based on Direct Synthesis of DNA on Paper.” Analytical Chemistry 88 (1) (January 5): 725–731. doi:10.1021/acs.analchem.5b02822.
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Abstract: This paper addresses a growing need in clinical diagnostics for parallel, multiplex analysis of biomarkers from small biological samples. It describes a new procedure for assembling microarrays of ssDNA and proteins on paper. This method starts with the synthesis of DNA oligonucleotides covalently linked to paper, and proceeds to generate DNA arrays capable of simultaneously capturing DNA, DNA-conjugated protein antigens, and DNA-conjugated antibodies. The synthesis of ssDNA oligonucleotides on paper is convenient and effective, with32% of the oligonucleotides cleaved and eluted from the paper substrate being full-length by HPLC for a 32-mer. These ssDNA arrays can be used to detect fluorophore-linked DNA oligonucleotides in solution, and as the basis for DNA-directed assembly of microarrays of DNA-conjugated capture antibodies on paper, detect protein antigens by sandwich ELISAs. Paper-anchored ssDNA arrays with different sequences can be used to assemble paper-based devices capable of detecting DNA and antibodies in the same device, and enable simple microfluidic paper-based devices.
Published Version: 10.1021/acs.analchem.5b02822
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at
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