Image-guided transplantation of single cells in the bone marrow of live animals

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Image-guided transplantation of single cells in the bone marrow of live animals

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Title: Image-guided transplantation of single cells in the bone marrow of live animals
Author: Turcotte, Raphaël; Alt, Clemens; Runnels, Judith M.; Ito, Kyoko; Wu, Juwell W.; Zaher, Walid; Mortensen, Luke J.; Silberstein, Lev; Côté, Daniel C.; Kung, Andrew L.; Ito, Keisuke; Lin, Charles P.

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Citation: Turcotte, R., C. Alt, J. M. Runnels, K. Ito, J. W. Wu, W. Zaher, L. J. Mortensen, et al. 2017. “Image-guided transplantation of single cells in the bone marrow of live animals.” Scientific Reports 7 (1): 3875. doi:10.1038/s41598-017-02896-6. http://dx.doi.org/10.1038/s41598-017-02896-6.
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Abstract: Transplantation of a single hematopoietic stem cell is an important method for its functional characterization, but the standard transplantation protocol relies on cell homing to the bone marrow after intravenous injection. Here, we present a method to transplant single cells directly into the bone marrow of live mice. We developed an optical platform that integrates a multiphoton microscope with a laser ablation unit for microsurgery and an optical tweezer for cell micromanipulation. These tools allow image-guided single cell transplantation with high spatial control. The platform was used to deliver single hematopoietic stem cells. The engraftment of transplants was tracked over time, illustrating that the technique can be useful for studying both normal and malignant stem cells in vivo.
Published Version: doi:10.1038/s41598-017-02896-6
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5478633/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:33490892
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