Hu, Ming, Ke Deng, Siddarth Selvaraj, Zhaohui Qin, Bing Ren, and Jun S. Liu. 2012. “HiCNorm: Removing Biases in Hi-C Data via Poisson Regression.” Bioinformatics 28 (23) (September 27): 3131–3133. doi:10.1093/bioinformatics/bts570.
We propose a parametric model, HiCNorm, to remove systematic biases in the raw Hi-C contact maps, resulting in a simple, fast, yet accurate normalization procedure. Compared to the existing Hi-C normalization method developed by Yaffe and Tanay, HiCNorm has fewer parameters, runs >1,000 times faster, and achieves higher reproducibility.