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dc.contributor.authorO'Rourke, Caitlin
dc.contributor.authorShelton, Georgia
dc.contributor.authorHutcheson, Joshua
dc.contributor.authorBurke, Megan F.
dc.contributor.authorMartyn, Trejeeve
dc.contributor.authorThayer, Timothy E.
dc.contributor.authorShakartzi, Hannah R.
dc.contributor.authorBuswell, Mary D.
dc.contributor.authorTainsh, Robert
dc.contributor.authorYu, Binglan
dc.contributor.authorBagchi, Aranya
dc.contributor.authorRhee, David Kwan
dc.contributor.authorWu, Connie
dc.contributor.authorDerwall, Matthias
dc.contributor.authorBuys, Emmanuel
dc.contributor.authorYu, Paul B.
dc.contributor.authorBloch, Kenneth
dc.contributor.authorAikawa, Elena
dc.contributor.authorBloch, Donald Bendit
dc.contributor.authorMalhotra, Rajeev
dc.date.accessioned2017-10-05T20:34:09Z
dash.embargo.terms2018-06-01
dc.date.issued2016
dc.identifierQuick submit: 2017-06-01T19:54:13-0400
dc.identifier.citationO’Rourke, Caitlin, Georgia Shelton, Joshua D. Hutcheson, Megan F. Burke, Trejeeve Martyn, Timothy E. Thayer, Hannah R. Shakartzi, et al. 2016. “Calcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammation.” Journal of Visualized Experiments (111) (May 31). doi:10.3791/54017.en_US
dc.identifier.issn1940-087Xen_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34070525
dc.description.abstractCardiovascular disease is the leading cause of morbidity and mortality in the world. Atherosclerotic plaques, consisting of lipid-laden macrophages and calcification, develop in the coronary arteries, aortic valve, aorta, and peripheral conduit arteries and are the hallmark of cardiovascular disease. In humans, imaging with computed tomography allows for the quantification of vascular calcification; the presence of vascular calcification is a strong predictor of future cardiovascular events. Development of novel therapies in cardiovascular disease relies critically on improving our understanding of the underlying molecular mechanisms of atherosclerosis. Advancing our knowledge of atherosclerotic mechanisms relies on murine and cell-based models. Here, a method for imaging aortic calcification and macrophage infiltration using two spectrally distinct near-infrared fluorescent imaging probes is detailed. Near-infrared fluorescent imaging allows for the ex vivo quantification of calcification and macrophage accumulation in the entire aorta and can be used to further our understanding of the mechanistic relationship between inflammation and calcification in atherosclerosis. Additionally, a method for isolating and culturing animal aortic vascular smooth muscle cells and a protocol for inducing calcification in cultured smooth muscle cells from either murine aortas or from human coronary arteries is described. This in vitro method of modeling vascular calcification can be used to identify an characterize the signaling pathways likely important for the development of vascular disease, in the hopes of discovering novel targets for therapy.en_US
dc.language.isoen_USen_US
dc.publisherMyJove Corporationen_US
dc.relation.isversionof10.3791/54017en_US
dc.relation.hasversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4927747/en_US
dash.licenseLAA
dc.subjectvascular calcificationen_US
dc.subjectMedicineen_US
dc.subjectIssue 111en_US
dc.subjectsmooth muscle cellen_US
dc.subjectmatrix gla proteinen_US
dc.subjectatherosclerosisen_US
dc.subjectmacrophageen_US
dc.subjectnear-infrared fluorescent imagingen_US
dc.subjectvascular inflammationen_US
dc.titleCalcification of Vascular Smooth Muscle Cells and Imaging of Aortic Calcification and Inflammationen_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-01T23:52:54Z
dc.description.versionAccepted Manuscripten_US
dc.relation.journalJournal of Visualized Experimentsen_US
dash.depositing.authorBuys, Emmanuel
dc.date.available2018-06-01T07:31:04Z
dc.identifier.doi10.3791/54017*
workflow.legacycommentscat.completeen_US
dash.authorsorderedfalse
dash.contributor.affiliatedBagchi, Aranya
dash.contributor.affiliatedHutcheson, Joshua
dash.contributor.affiliatedMalhotra, Rajeev
dash.contributor.affiliatedYu, Binglan
dash.contributor.affiliatedAikawa, Elena
dash.contributor.affiliatedYu, Paul
dash.contributor.affiliatedRhee, David Kwan
dash.contributor.affiliatedWu, Connie
dash.contributor.affiliatedBloch, Donald
dash.contributor.affiliatedTainsh, Robert
dash.contributor.affiliatedBuys, Emmanuel
dash.contributor.affiliatedBloch, Kenneth


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