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dc.contributor.authorTahiliani, Mamta V.
dc.contributor.authorKoh, Kian Peng
dc.contributor.authorShen, Yinghua
dc.contributor.authorPastor, William Abraham
dc.contributor.authorBandukwala, Hozefa S
dc.contributor.authorBrudno, Yevgeny
dc.contributor.authorAgarwal, Suneet
dc.contributor.authorIyer, Lakshminarayan M.
dc.contributor.authorLiu, David Ruchien
dc.contributor.authorAravind, L
dc.contributor.authorRao, Anjana
dc.date.accessioned2009-11-20T19:52:06Z
dc.date.issued2009
dc.identifier.citationTahiliani, Mamta, Kian Peng Koh, Yinghua Shen, William A. Pastor, Hozefa Bandukwala, Yevgeny Brudno, Suneet Agarwal, Lakshminarayan M. Iyer, David R. Liu, L. Aravind, and Anjana Rao. 2009. Conversion of 5-methylcytosine to 5-hydroxymethylcytosine in mammalian DNA by MLL partner TET1. Science 324(5929): 930-935.en_US
dc.identifier.issn0193-4511en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:3415331
dc.description.abstractDNA cytosine methylation is crucial for retrotransposon silencing and mammalian development. In a computational search for enzymes that could modify 5-methylcytosine (5mC), we identified TET proteins as mammalian homologs of the trypanosome proteins JBP1 and JBP2, which have been proposed to oxidize the 5-methyl group of thymine. We show here that TET1, a fusion partner of the MLL gene in acute myeloid leukemia, is a 2-oxoglutarate (2OG)- and Fe(II)-dependent enzyme that catalyzes conversion of 5mC to 5-hydroxymethylcytosine (hmC) in cultured cells and in vitro. hmC is present in the genome of mouse embryonic stem cells, and hmC levels decrease upon RNA interference–mediated depletion of TET1. Thus, TET proteins have potential roles in epigenetic regulation through modification of 5mC to hmC.en_US
dc.description.sponsorshipChemistry and Chemical Biologyen_US
dc.language.isoen_USen_US
dc.publisherAmerican Association for the Advancement of Scienceen_US
dc.relation.isversionofhttp://dx.doi.org/10.1126/science.1170116en_US
dc.relation.hasversionhttp://evolve.harvard.edu/55-hmC-Science.pdfen_US
dash.licenseOAP
dc.titleConversion of 5-Methylcytosine to 5-Hydroxymethylcytosine in Mammalian DNA by the MLL Partner TET1en_US
dc.typeJournal Articleen_US
dc.description.versionAccepted Manuscripten_US
dc.relation.journalScienceen_US
dash.depositing.authorLiu, David Ruchien
dc.date.available2009-11-20T19:52:06Z
dc.identifier.doi10.1126/science.1170116*
dash.contributor.affiliatedKoh, Kian Peng
dash.contributor.affiliatedBandukwala, Hozefa S
dash.contributor.affiliatedBrudno, Yevgeny
dash.contributor.affiliatedAgarwal, Suneet
dash.contributor.affiliatedPastor, William Abraham
dash.contributor.affiliatedTahiliani, Mamta V.
dash.contributor.affiliatedRao, Anjana


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