A Rotary Motor Drives Flavobacterium Gliding

DSpace/Manakin Repository

A Rotary Motor Drives Flavobacterium Gliding

Citable link to this page


Title: A Rotary Motor Drives Flavobacterium Gliding
Author: Shrivastava, Abhishek; Lele, Pushkar Prakash; Berg, Howard Curtis

Note: Order does not necessarily reflect citation order of authors.

Citation: Shrivastava, Abhishek, Pushkar P. Lele, and Howard C. Berg. 2015. “A Rotary Motor Drives Flavobacterium Gliding.” Current Biology 25, no. 3: 338–341. doi:10.1016/j.cub.2014.11.045.
Full Text & Related Files:
Abstract: Cells of Flavobacterium johnsoniae, a rod-shaped bacterium devoid of pili or flagella, glide over glass at speeds of 2–4 μm/s [ 1 ]. Gliding is powered by a protonmotive force [ 2 ], but the machinery required for this motion is not known. Usually, cells move along straight paths, but sometimes they exhibit a reciprocal motion, attach near one pole and flip end over end, or rotate. This behavior is similar to that of a Cytophaga species described earlier [ 3 ]. Development of genetic tools for F. johnsoniae led to discovery of proteins involved in gliding [ 4 ]. These include the surface adhesin SprB that forms filaments about 160 nm long by 6 nm in diameter, which, when labeled with a fluorescent antibody [ 2 ] or a latex bead [ 5 ], are seen to move longitudinally down the length of a cell, occasionally shifting positions to the right or the left. Evidently, interaction of these filaments with a surface produces gliding. To learn more about the gliding motor, we sheared cells to reduce the number and size of SprB filaments and tethered cells to glass by adding anti-SprB antibody. Cells spun about fixed points, mostly counterclockwise, rotating at speeds of 1 Hz or more. The torques required to sustain such speeds were large, comparable to those generated by the flagellar rotary motor. However, we found that a gliding motor runs at constant speed rather than at constant torque. Now, there are three rotary motors powered by protonmotive force: the bacterial flagellar motor, the Fo ATP synthase, and the gliding motor.
Published Version: doi:10.1016/j.cub.2014.11.045
Terms of Use: This article is made available under the terms and conditions applicable to Open Access Policy Articles, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#OAP
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34309452
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search