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dc.contributor.authorZhang, Sicaien_US
dc.contributor.authorMasuyer, Geoffreyen_US
dc.contributor.authorZhang, Jieen_US
dc.contributor.authorShen, Yien_US
dc.contributor.authorLundin, Danielen_US
dc.contributor.authorHenriksson, Lindaen_US
dc.contributor.authorMiyashita, Shin-Ichiroen_US
dc.contributor.authorMartínez-Carranza, Markelen_US
dc.contributor.authorDong, Minen_US
dc.contributor.authorStenmark, Pålen_US
dc.date.accessioned2017-11-21T20:42:34Z
dc.date.issued2017en_US
dc.identifier.citationZhang, Sicai, Geoffrey Masuyer, Jie Zhang, Yi Shen, Daniel Lundin, Linda Henriksson, Shin-Ichiro Miyashita, Markel Martínez-Carranza, Min Dong, and Pål Stenmark. 2017. “Identification and characterization of a novel botulinum neurotoxin.” Nature Communications 8 (1): 14130. doi:10.1038/ncomms14130. http://dx.doi.org/10.1038/ncomms14130.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34375147
dc.description.abstractBotulinum neurotoxins are known to have seven serotypes (BoNT/A–G). Here we report a new BoNT serotype, tentatively named BoNT/X, which has the lowest sequence identity with other BoNTs and is not recognized by antisera against known BoNTs. Similar to BoNT/B/D/F/G, BoNT/X cleaves vesicle-associated membrane proteins (VAMP) 1, 2 and 3, but at a novel site (Arg66-Ala67 in VAMP2). Remarkably, BoNT/X is the only toxin that also cleaves non-canonical substrates VAMP4, VAMP5 and Ykt6. To validate its activity, a small amount of full-length BoNT/X was assembled by linking two non-toxic fragments using a transpeptidase (sortase). Assembled BoNT/X cleaves VAMP2 and VAMP4 in cultured neurons and causes flaccid paralysis in mice. Thus, BoNT/X is a novel BoNT with a unique substrate profile. Its discovery posts a challenge to develop effective countermeasures, provides a novel tool for studying intracellular membrane trafficking, and presents a new potential therapeutic toxin for modulating secretions in cells.en
dc.language.isoen_USen
dc.publisherNature Publishing Groupen
dc.relation.isversionofdoi:10.1038/ncomms14130en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5543303/pdf/en
dash.licenseLAAen_US
dc.titleIdentification and characterization of a novel botulinum neurotoxinen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalNature Communicationsen
dash.depositing.authorZhang, Sicaien_US
dc.date.available2017-11-21T20:42:34Z
dc.identifier.doi10.1038/ncomms14130*
dash.authorsorderedfalse
dash.contributor.affiliatedShen, Yi
dash.contributor.affiliatedMiyashita, Shin-Ichiro
dash.contributor.affiliatedZhang, Sicai
dash.contributor.affiliatedDong, Min


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