DNA methylation of candidate genes in peripheral blood from patients with type 2 diabetes or the metabolic syndrome

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DNA methylation of candidate genes in peripheral blood from patients with type 2 diabetes or the metabolic syndrome

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Title: DNA methylation of candidate genes in peripheral blood from patients with type 2 diabetes or the metabolic syndrome
Author: van Otterdijk, Sanne D.; Binder, Alexandra M.; Szarc vel Szic, Katarzyna; Schwald, Julia; Michels, Karin B.

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Citation: van Otterdijk, Sanne D., Alexandra M. Binder, Katarzyna Szarc vel Szic, Julia Schwald, and Karin B. Michels. 2017. “DNA methylation of candidate genes in peripheral blood from patients with type 2 diabetes or the metabolic syndrome.” PLoS ONE 12 (7): e0180955. doi:10.1371/journal.pone.0180955. http://dx.doi.org/10.1371/journal.pone.0180955.
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Abstract: Introduction: The prevalence of type 2 diabetes (T2D) and the metabolic syndrome (MetS) is increasing and several studies suggested an involvement of DNA methylation in the development of these metabolic diseases. This study was designed to investigate if differential DNA methylation in blood can function as a biomarker for T2D and/or MetS. Methods: Pyrosequencing analyses were performed for the candidate genes KCNJ11, PPARγ, PDK4, KCNQ1, SCD1, PDX1, FTO and PEG3 in peripheral blood leukocytes (PBLs) from 25 patients diagnosed with only T2D, 9 patients diagnosed with T2D and MetS and 11 control subjects without any metabolic disorders. Results: No significant differences in gene-specific methylation between patients and controls were observed, although a trend towards significance was observed for PEG3. Differential methylation was observed between the groups in 4 out of the 42 single CpG loci located in the promoters regions of the genes FTO, KCNJ11, PPARγ and PDK4. A trend towards a positive correlation was observed for PEG3 methylation with HDL cholesterol levels. Discussion Altered levels of DNA methylation in PBLs of specific loci might serve as a biomarker for T2D or MetS, although further investigation is required.
Published Version: doi:10.1371/journal.pone.0180955
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5519053/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34375338
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