Characterization of Langerin-Expressing Dendritic Cell Subsets in the Normal Cornea
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Hattori, Takaaki
Lee, Hyunsoo
Ueno, Hiroki
Kaplan, Daniel H.
Saban, Daniel R.
Published Version
https://doi.org/10.1167/iovs.10-6741Metadata
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Hattori, Takaaki, Sunil K. Chauhan, Hyunsoo Lee, Hiroki Ueno, Reza Dana, Daniel H. Kaplan, and Daniel R. Saban. 2011. “Characterization of Langerin-Expressing Dendritic Cell Subsets in the Normal Cornea.” Investigative Opthalmology & Visual Science 52 (7) (June 28): 4598. doi:10.1167/iovs.10-6741.Abstract
Purpose.In addition to Langerhans cells (LCs), other dendritic cells (CD11c+) have recently been shown to express Langerin (c-type lectin). In skin, (non-LC) Langerin+ dendritic cells initiate adaptive immunity. However, whether such dendritic cells (DC) reside in the cornea, an immune-privileged tissue, is unknown.
Methods.
Normal C57BL/6 corneas were harvested for qRT-PCR analyses of Langerin expression in the epithelium versus stroma. Immunohistochemistry for Langerin was also performed. Single-cell preparations of epithelium versus stroma were FACS analyzed for CD11c, CD11b, and CD103 expression. Fluorescence microscopy of corneas from muLangerin-eGFP mice (in which all CD11c+ Langerin+ cells express eGFP), huLangerin-DTA mice (only LCs are constitutively deleted), and huLangerin-Cre eYFP-flox (only LCs express eYFP) was performed.
Results.
qRT-PCR, immunohistochemistry, and FACS analysis identified CD11c+ Langerin+ cells in the epithelium and stroma. Similarly, corneas of muLangerin-eGFP mice contained eGFP+ cells in the epithelium and stroma. However, FACS analysis indicated phenotypically differing CD11c+ Langerin+ populations in the epithelium (CD11blowCD103low) versus stroma (CD11b+CD103low). Additionally, corneas from huLangerin-DTA mice were devoid of Langerin+ cells in the epithelium but were detectable in the stroma. In corneas from huLangerin-Cre eYFP-flox, eYFP+ cells were detectable in the epithelium but not in the stroma.
Conclusions.
The normal corneal epithelium is endowed with CD11c+ Langerin+ cells that are LCs, whereas the stroma is endowed with a separate population of (non-LC) Langerin+ DCs. These findings should henceforth facilitate the examination of Langerin-expressing DC subsets in the immunopathogeneses of conditions such as keratoconjunctivitis sicca, allergic keratoconjunctivitis, and corneal allograft rejection.
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https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3175952/Citable link to this page
http://nrs.harvard.edu/urn-3:HUL.InstRepos:34387113
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