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dc.contributor.authorQian, Ying
dc.contributor.authorLeong, Fee-Lai
dc.contributor.authorKazlauskas, Andrius
dc.contributor.authorDana, Reza
dc.date.accessioned2017-11-27T20:23:16Z
dc.date.issued2004
dc.identifierQuick submit: 2017-06-18T19:05:05-0400
dc.identifier.citationQian, Ying, Fee-Lai Leong, Andrius Kazlauskas, and M. Reza Dana. 2004. “Ex Vivo Adenovirus-Mediated Gene Transfer to Corneal Graft Endothelial Cells in Mice.” Investigative Opthalmology & Visual Science 45 (7) (July 1): 2187. doi:10.1167/iovs.03-0901.en_US
dc.identifier.issn1552-5783en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34388128
dc.description.abstractpurpose. Genetic modulation of donor tissue before corneal transplantation may have the potential to modulate alloimmunity and/or to prevent corneal endothelial cell death. This study was conducted to optimize adenovirus-mediated gene transfer to donor corneal endothelium and to delineate the kinetics of marker gene expression in syngeneic and allogeneic corneal grafts. methods. BALB/c mouse corneas were incubated with replication-deficient adenovirus encoding green fluorescent protein (GFP) or empty vector ex vivo at a dose of 6 × 107 or 6 × 106 PFU at temperatures of 4°C or 37°C. After ex vivo infection, the donor corneas were transplanted orthotopically to BALB/c or C57BL/6 recipients. After transplantation, localization of GFP in the grafts was determined in cryosections of enucleated eyes, and GFP expression in the grafts was visualized in vivo by using epifluorescence microscopy over 12 weeks. All grafts were evaluated clinically by slit lamp biomicroscopy. results. GFP expression was found to be restricted to the corneal endothelium. In vivo expression of GFP in syngeneic corneal grafts was demonstrated for up to 12 weeks. Syngeneic grafts incubated with the vector at 4°C exhibited a more extensive and longer duration of expression of green fluorescence than grafts incubated at 37°C. Moreover, the syngeneic grafts infected at 4°C maintained their transparency, whereas those infected at 37°C displayed a high degree of opacity. Corneal allogeneic grafts infected with a low dose of the vector displayed longer GFP expression and graft survival than the allogeneic grafts infected with a high dose of the viral vector. conclusions. Adenoviral vector can selectively and efficiently deliver exogenous gene(s) to the endothelium of corneal grafts during hypothermic organ preservation. Gene expression is retained in vivo in corneal syngeneic grafts for longer periods than are allogeneic grafts.en_US
dc.language.isoen_USen_US
dc.publisherAssociation for Research in Vision and Ophthalmology (ARVO)en_US
dc.relation.isversionof10.1167/iovs.03-0901en_US
dash.licenseLAA
dc.titleEx Vivo Adenovirus-Mediated Gene Transfer to Corneal Graft Endothelial Cells in Miceen_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-18T23:05:06Z
dc.description.versionVersion of Recorden_US
dc.relation.journalInvestigative Opthalmology & Visual Scienceen_US
dash.depositing.authorDana, Reza
dc.date.available2004
dc.date.available2017-11-27T20:23:16Z
dc.identifier.doi10.1167/iovs.03-0901*
workflow.legacycommentscat.completeen_US
dash.contributor.affiliatedDana, Reza


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