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dc.contributor.authorSakai, Rieko
dc.contributor.authorKinouchi, Tadatoshi
dc.contributor.authorKawamoto, Shoko
dc.contributor.authorDana, Reza
dc.contributor.authorHamamoto, Toshirou
dc.contributor.authorTsuru, Tadahiko
dc.contributor.authorOkubo, Kousaku
dc.contributor.authorYamagami, Satoru
dc.date.accessioned2017-11-27T20:38:45Z
dc.date.issued2017-11-27
dc.identifierQuick submit: 2017-06-18T18:56:19-0400
dc.identifier.citationSakai,Reiko, Tadatoshi Kinouchi, Shoko Kawamoto, M. Reza Dana, Toshirou Hamamoto, Tadahiko Tsuru, Kousaku Okubo and Satoru Yamagami. 2002. "Construction of Human Corneal Endothelial cDNA Library and Identification of Novel Active Genes." Investigative Ophthalmology & Visual Science 43, no.6:1749-56.en_US
dc.identifier.issn0146-0404en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34388134
dc.description.abstractpurpose. To describe genes expressed in human corneal endothelial cells and identify novel genes. methods. Sixteen human donor corneas that had no history of corneal disease, infection, or intraocular surgery were used within 7 days of death. Total RNA was extracted from corneal endothelial cells with attached Descemet membranes. A 3′-directed cDNA library was constructed from mRNA by using a pUC19-based primer. These sequences were compared with each other to determine their frequency and were searched against GenBank for identification. To identify novel specific and abundant transcript genes in corneal endothelial cells, the novel genes were compared with an expressed sequence tag database, the expected sequence extended, and 5′ rapid amplification of cDNA ends–polymerase chain reaction cloning performed. results. The human corneal endothelial cDNA library showed that the most abundant transcript was prostaglandin D2 synthase. The remaining transcript genes that were present in abundance consisted of lactate dehydrogenase-A, gene signature (GS) 3582, which is a novel gene without a known function, and matrix Gla protein. The full-length sequence of GS3582 showed similarity to genes obtained in ovary and testis. conclusions. A human corneal endothelial cDNA library was constructed. An expression profile of corneal endothelium provides probes to monitor physiologic and pathologic conditions of this tissue in terms of gene expression.en_US
dc.language.isoen_USen_US
dc.publisherAssociation for Research in Vision and Ophthalmologyen_US
dc.relation.isversionofhttp://iovs.arvojournals.org/article.aspx?articleid=2123315en_US
dash.licenseLAA
dc.titleConstruction of Human Corneal Endothelial cDNA Library and Identification of Novel Active Genesen_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-18T22:56:21Z
dc.description.versionVersion of Recorden_US
dc.relation.journalInvestigative Ophthalmology & Visual Scienceen_US
dash.depositing.authorDana, Reza
dc.date.available2002
dc.date.available2017-11-27T20:38:45Z
dash.contributor.affiliatedDana, Reza


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