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dc.contributor.authorShim, Jongwoo
dc.contributor.authorPark, Changhun
dc.contributor.authorLee, Hyun Soo
dc.contributor.authorPark, Min Soo
dc.contributor.authorLim, Hyung Taek
dc.contributor.authorChauhan, Sunil Kumar
dc.contributor.authorDana, Reza
dc.contributor.authorLee, Hyon
dc.contributor.authorLee, Hyung Keun
dc.date.accessioned2017-12-05T15:22:54Z
dc.date.issued2012
dc.identifierQuick submit: 2017-06-18T21:03:43-0400
dc.identifier.citationShim, Jongwoo, Changhun Park, Hyun Soo Lee, Min Soo Park, Hyung Taek Lim, Sunil Chauhan, Reza Dana, Hyon Lee, and Hyung Keun Lee. 2012. “Change in Prostaglandin Expression Levels and Synthesizing Activities in Dry Eye Disease.” Ophthalmology 119 (11) (November): 2211–2219. doi:10.1016/j.ophtha.2012.05.038.en_US
dc.identifier.issn0161-6420en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34428276
dc.description.abstractObjective To investigate the expression level of prostaglandins (PGs) and their de novo synthesis in dry eye (DE) disease. Design Cross-sectional case-control study and in vivo mouse experimental study. Participants Forty-six eyes from 23 DE patients and 33 eyes from 17 age- and sex-matched controls were studied. Also, DE-induced murine eyes were compared with control eyes. Methods Patients completed a symptom questionnaire using a 100-mm visual analog scale (VAS). Nanoliquid chromatography tandem mass spectrometry was used for the quantification of PGE2 and PGD2. A DE disease environmental chamber was used to induce DE in mice. One week after induction, enzyme expressions of cyclooxygenase-1, cyclooxygenase-2 (COX-2), PG E synthase (PGES), and PG D synthase (PGDS) in the lacrimal glands, meibomian glands, and corneas were examined using immunohistochemistry and quantitative real-time polymerase chain reaction (qRT-PCR). Main Outcome Measures The mean PGE2 and PGD2 levels in the tears of DE patients were measured and compared with symptom severity scores. Immunohistochemistry staining patterns and qRT-PCR data of DE mice were quantified. Results The mean PGE2 level in the tears of DE patients (2.72±3.42 ng/ml) was significantly higher than that in the control group (0.88±0.83 ng/ml; P = 0.003). However, the mean PGD2 level in the tears of DE patients (0.11 ±0.22 ng/ml) was significantly lower (0.91 ±3.28 ng/ml; P = 0.028). The mean PGE2-to-PGD2 ratio correlated strongly with VAS scoring (P = 0.008). In DE mice, COX-2 mRNA was significantly higher in ocular surface tissue and lacrimal glands. Furthermore, PGES mRNA was significantly higher in ocular surface tissue, whereas PGDS mRNA was decreased. Immunohistochemistry staining showed elevated COX-2 expression in the lacrimal glands, meibomian glands, corneas, and conjunctivas. Furthermore, PGES expression was found in periductal infiltrated cells of the lacrimal glands and conjunctival epithelium. Also, PGDS expression was decreased in meibomian glands and increased focally in the conjunctival epithelium. Conclusions A reciprocal change in PGE2 and PGD2 levels was found in the tears of DE patients, which correlated with patients’ symptom scores. These clinical results were supported by increased COX-2 and PGES expression levels found in tear-producing tissues of DE mice. Financial Disclosure(s) The author(s) have no proprietary or commercial interest in any materials discussed in this article.en_US
dc.language.isoen_USen_US
dc.publisherElsevier BVen_US
dc.relation.isversionof10.1016/j.ophtha.2012.05.038en_US
dc.relation.hasversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC3689228/en_US
dash.licenseLAA
dc.titleChange in Prostaglandin Expression Levels and Synthesizing Activities in Dry Eye Diseaseen_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-19T01:03:49Z
dc.description.versionAccepted Manuscripten_US
dc.relation.journalOphthalmologyen_US
dash.depositing.authorDana, Reza
dc.date.available2012
dc.date.available2017-12-05T15:22:54Z
dc.identifier.doi10.1016/j.ophtha.2012.05.038*
workflow.legacycommentscat.completeen_US
dash.authorsorderedfalse
dash.contributor.affiliatedChauhan, Sunil
dash.contributor.affiliatedDana, Reza


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