CryoEM structure of MxB reveals a novel oligomerization interface critical for HIV restriction

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CryoEM structure of MxB reveals a novel oligomerization interface critical for HIV restriction

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Title: CryoEM structure of MxB reveals a novel oligomerization interface critical for HIV restriction
Author: Alvarez, Frances J. D.; He, Shaoda; Perilla, Juan R.; Jang, Sooin; Schulten, Klaus; Engelman, Alan N.; Scheres, Sjors H. W.; Zhang, Peijun

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Citation: Alvarez, Frances J. D., Shaoda He, Juan R. Perilla, Sooin Jang, Klaus Schulten, Alan N. Engelman, Sjors H. W. Scheres, and Peijun Zhang. 2017. “CryoEM structure of MxB reveals a novel oligomerization interface critical for HIV restriction.” Science Advances 3 (9): e1701264. doi:10.1126/sciadv.1701264. http://dx.doi.org/10.1126/sciadv.1701264.
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Abstract: Human dynamin–like, interferon-induced myxovirus resistance 2 (Mx2 or MxB) is a potent HIV-1 inhibitor. Antiviral activity requires both the amino-terminal region of MxB and protein oligomerization, each of which has eluded structural determination due to difficulties in protein preparation. We report that maltose binding protein–fused, full-length wild-type MxB purifies as oligomers and further self-assembles into helical arrays in physiological salt. Guanosine triphosphate (GTP), but not guanosine diphosphate, binding results in array disassembly, whereas subsequent GTP hydrolysis allows its reformation. Using cryo-electron microscopy (cryoEM), we determined the MxB assembly structure at 4.6 Å resolution, representing the first near-atomic resolution structure in the mammalian dynamin superfamily. The structure revealed previously described and novel MxB assembly interfaces. Mutational analyses demonstrated a critical role for one of the novel interfaces in HIV-1 restriction.
Published Version: doi:10.1126/sciadv.1701264
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5600524/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492005
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