A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development

DSpace/Manakin Repository

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development

Citable link to this page

 

 
Title: A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development
Author: Ciarlo, Christie; Kaufman, Charles K; Kinikoglu, Beste; Michael, Jonathan; Yang, Song; D′Amato, Christopher; Blokzijl-Franke, Sasja; den Hertog, Jeroen; Schlaeger, Thorsten M; Zhou, Yi; Liao, Eric; Zon, Leonard I

Note: Order does not necessarily reflect citation order of authors.

Citation: Ciarlo, C., C. K. Kaufman, B. Kinikoglu, J. Michael, S. Yang, C. D′Amato, S. Blokzijl-Franke, et al. 2017. “A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development.” eLife 6 (1): e29145. doi:10.7554/eLife.29145. http://dx.doi.org/10.7554/eLife.29145.
Full Text & Related Files:
Abstract: The neural crest is a dynamic progenitor cell population that arises at the border of neural and non-neural ectoderm. The inductive roles of FGF, Wnt, and BMP at the neural plate border are well established, but the signals required for subsequent neural crest development remain poorly characterized. Here, we conducted a screen in primary zebrafish embryo cultures for chemicals that disrupt neural crest development, as read out by crestin:EGFP expression. We found that the natural product caffeic acid phenethyl ester (CAPE) disrupts neural crest gene expression, migration, and melanocytic differentiation by reducing Sox10 activity. CAPE inhibits FGF-stimulated PI3K/Akt signaling, and neural crest defects in CAPE-treated embryos are suppressed by constitutively active Akt1. Inhibition of Akt activity by constitutively active PTEN similarly decreases crestin expression and Sox10 activity. Our study has identified Akt as a novel intracellular pathway required for neural crest differentiation.
Published Version: doi:10.7554/eLife.29145
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5599238/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492086
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters