A microfluidic renal proximal tubule with active reabsorptive function

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A microfluidic renal proximal tubule with active reabsorptive function

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Title: A microfluidic renal proximal tubule with active reabsorptive function
Author: Vedula, Else M.; Alonso, José Luis; Arnaout, M. Amin; Charest, Joseph L.

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Citation: Vedula, Else M., José Luis Alonso, M. Amin Arnaout, and Joseph L. Charest. 2017. “A microfluidic renal proximal tubule with active reabsorptive function.” PLoS ONE 12 (10): e0184330. doi:10.1371/journal.pone.0184330. http://dx.doi.org/10.1371/journal.pone.0184330.
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Abstract: In the kidney, the renal proximal tubule (PT) reabsorbs solutes into the peritubular capillaries through active transport. Here, we replicate this reabsorptive function in vitro by engineering a microfluidic PT. The microfluidic PT architecture comprises a porous membrane with user-defined submicron surface topography separating two microchannels representing a PT filtrate lumen and a peritubular capillary lumen. Human PT epithelial cells and microvascular endothelial cells in respective microchannels created a PT-like reabsorptive barrier. Co-culturing epithelial and endothelial cells in the microfluidic architecture enhanced viability, metabolic activity, and compactness of the epithelial layer. The resulting tissue expressed tight junctions, kidney-specific morphology, and polarized expression of kidney markers. The microfluidic PT actively performed sodium-coupled glucose transport, which could be modulated by administration of a sodium-transport inhibiting drug. The microfluidic PT reproduces human physiology at the cellular and tissue levels, and measurable tissue function which can quantify kidney pharmaceutical efficacy and toxicity.
Published Version: doi:10.1371/journal.pone.0184330
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636065/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492146
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