Nuclear Wiskott–Aldrich syndrome protein co-regulates T cell factor 1-mediated transcription in T cells
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Kuznetsov, Nikolai V.
Almuzzaini, Bader
Kritikou, Joanna S.
Baptista, Marisa A. P.
Oliveira, Mariana M. S.
Keszei, Marton
Percipalle, Piergiorgio
Westerberg, Lisa S.
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https://doi.org/10.1186/s13073-017-0481-6Metadata
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Kuznetsov, Nikolai V., Bader Almuzzaini, Joanna S. Kritikou, Marisa A. P. Baptista, Mariana M. S. Oliveira, Marton Keszei, Scott B. Snapper, Piergiorgio Percipalle, and Lisa S. Westerberg. 2017. “Nuclear Wiskott–Aldrich syndrome protein co-regulates T cell factor 1-mediated transcription in T cells.” Genome Medicine 9 (1): 91. doi:10.1186/s13073-017-0481-6. http://dx.doi.org/10.1186/s13073-017-0481-6.Abstract
Background: The Wiskott–Aldrich syndrome protein (WASp) family of actin-nucleating factors are present in the cytoplasm and in the nucleus. The role of nuclear WASp for T cell development remains incompletely defined. Methods: We performed WASp chromatin immunoprecipitation and deep sequencing (ChIP-seq) in thymocytes and spleen CD4+ T cells. Results: WASp was enriched at genic and intergenic regions and associated with the transcription start sites of protein-coding genes. Thymocytes and spleen CD4+ T cells showed 15 common WASp-interacting genes, including the gene encoding T cell factor (TCF)12. WASp KO thymocytes had reduced nuclear TCF12 whereas thymocytes expressing constitutively active WASpL272P and WASpI296T had increased nuclear TCF12, suggesting that regulated WASp activity controlled nuclear TCF12. We identify a putative DNA element enriched in WASp ChIP-seq samples identical to a TCF1-binding site and we show that WASp directly interacted with TCF1 in the nucleus. Conclusions: These data place nuclear WASp in proximity with TCF1 and TCF12, essential factors for T cell development. Electronic supplementary material The online version of this article (doi:10.1186/s13073-017-0481-6) contains supplementary material, which is available to authorized users.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5660450/pdf/Terms of Use
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