ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones
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Author
Kawabe, Hiroshi
Mitkovski, Miso
Hirrlinger, Johannes
Opazo, Felipe
Nestvogel, Dennis
Kalla, Stefan
Fejtova, Anna
Verrier, Sophie E.
Bungers, Simon R.
Cooper, Benjamin H.
Varoqueaux, Frederique
Wang, Yun
Nehring, Ralf B.
Gundelfinger, Eckart D.
Rosenmund, Christian
Rizzoli, Silvio O.
Südhof, Thomas C.
Rhee, Jeong-Seop
Brose, Nils
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1083/jcb.201606086Metadata
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Kawabe, H., M. Mitkovski, P. S. Kaeser, J. Hirrlinger, F. Opazo, D. Nestvogel, S. Kalla, et al. 2017. “ELKS1 localizes the synaptic vesicle priming protein bMunc13-2 to a specific subset of active zones.” The Journal of Cell Biology 216 (4): 1143-1161. doi:10.1083/jcb.201606086. http://dx.doi.org/10.1083/jcb.201606086.Abstract
Presynaptic active zones (AZs) are unique subcellular structures at neuronal synapses, which contain a network of specific proteins that control synaptic vesicle (SV) tethering, priming, and fusion. Munc13s are core AZ proteins with an essential function in SV priming. In hippocampal neurons, two different Munc13s—Munc13-1 and bMunc13-2—mediate opposite forms of presynaptic short-term plasticity and thus differentially affect neuronal network characteristics. We found that most presynapses of cortical and hippocampal neurons contain only Munc13-1, whereas ∼10% contain both Munc13-1 and bMunc13-2. Whereas the presynaptic recruitment and activation of Munc13-1 depends on Rab3-interacting proteins (RIMs), we demonstrate here that bMunc13-2 is recruited to synapses by the AZ protein ELKS1, but not ELKS2, and that this recruitment determines basal SV priming and short-term plasticity. Thus, synapse-specific interactions of different Munc13 isoforms with ELKS1 or RIMs are key determinants of the molecular and functional heterogeneity of presynaptic AZs.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5379939/pdf/Terms of Use
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