Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities
Palmer, Christine D.
Hess, Paul R.
Matthews, Philippa C.
Goulder, Philip J. R.Note: Order does not necessarily reflect citation order of authors.
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CitationLeitman, E. M., C. D. Palmer, S. Buus, F. Chen, L. Riddell, S. Sims, P. Klenerman, et al. 2017. “Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities.” PLoS ONE 12 (10): e0184496. doi:10.1371/journal.pone.0184496. http://dx.doi.org/10.1371/journal.pone.0184496.
AbstractAntigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers.
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