dc.contributor.author | Leitman, Ellen M. | en_US |
dc.contributor.author | Palmer, Christine D. | en_US |
dc.contributor.author | Buus, Søren | en_US |
dc.contributor.author | Chen, Fabian | en_US |
dc.contributor.author | Riddell, Lynn | en_US |
dc.contributor.author | Sims, Stuart | en_US |
dc.contributor.author | Klenerman, Paul | en_US |
dc.contributor.author | Sáez-Cirión, Asier | en_US |
dc.contributor.author | Walker, Bruce D. | en_US |
dc.contributor.author | Hess, Paul R. | en_US |
dc.contributor.author | Altfeld, Marcus | en_US |
dc.contributor.author | Matthews, Philippa C. | en_US |
dc.contributor.author | Goulder, Philip J. R. | en_US |
dc.date.accessioned | 2017-12-06T05:55:22Z | |
dc.date.issued | 2017 | en_US |
dc.identifier.citation | Leitman, E. M., C. D. Palmer, S. Buus, F. Chen, L. Riddell, S. Sims, P. Klenerman, et al. 2017. “Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities.” PLoS ONE 12 (10): e0184496. doi:10.1371/journal.pone.0184496. http://dx.doi.org/10.1371/journal.pone.0184496. | en |
dc.identifier.issn | | en |
dc.identifier.uri | http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492405 | |
dc.description.abstract | Antigen-specific T-cells are highly variable, spanning potent antiviral efficacy and damaging auto-reactivity. In virus infections, identifying the most efficacious responses is critical to vaccine design. However, current methods depend on indirect measures or on ex vivo expanded CTL clones. We here describe a novel application of cytotoxic saporin-conjugated tetramers to kill antigen-specific T-cells without significant off-target effects. The relative efficacy of distinct antiviral CD8+ T-cell specificity can be directly assessed via antigen-specific CD8+ T-cell depletion. The utility of these reagents is demonstrated here in identifying the CD8+ T-cell specificity most effective in preventing HIV progression in HIV-infected HLA-B*27-positive immune controllers. | en |
dc.language.iso | en_US | en |
dc.publisher | Public Library of Science | en |
dc.relation.isversionof | doi:10.1371/journal.pone.0184496 | en |
dc.relation.hasversion | http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5636067/pdf/ | en |
dash.license | LAA | en_US |
dc.subject | Biology and Life Sciences | en |
dc.subject | Cell Biology | en |
dc.subject | Cellular Types | en |
dc.subject | Animal Cells | en |
dc.subject | Blood Cells | en |
dc.subject | White Blood Cells | en |
dc.subject | T Cells | en |
dc.subject | Immune Cells | en |
dc.subject | Immunology | en |
dc.subject | Medicine and Health Sciences | en |
dc.subject | Biology and life sciences | en |
dc.subject | Cell biology | en |
dc.subject | Cellular types | en |
dc.subject | Animal cells | en |
dc.subject | Blood cells | en |
dc.subject | White blood cells | en |
dc.subject | T cells | en |
dc.subject | Cytotoxic T cells | en |
dc.subject | Immune cells | en |
dc.subject | Medicine and health sciences | en |
dc.subject | Specimen Preparation and Treatment | en |
dc.subject | Staining | en |
dc.subject | Cell Staining | en |
dc.subject | Microbiology | en |
dc.subject | Medical Microbiology | en |
dc.subject | Microbial Pathogens | en |
dc.subject | Viral Pathogens | en |
dc.subject | Immunodeficiency Viruses | en |
dc.subject | HIV | en |
dc.subject | Pathology and Laboratory Medicine | en |
dc.subject | Pathogens | en |
dc.subject | Organisms | en |
dc.subject | Viruses | en |
dc.subject | RNA viruses | en |
dc.subject | Retroviruses | en |
dc.subject | Lentivirus | en |
dc.subject | Virology | en |
dc.subject | Viral Replication | en |
dc.subject | Immunologic Techniques | en |
dc.subject | Immunoassays | en |
dc.subject | Enzyme-Linked Immunoassays | en |
dc.subject | Viral Transmission and Infection | en |
dc.subject | Viral Load | en |
dc.subject | Cell Physiology | en |
dc.subject | Cell Binding | en |
dc.title | Saporin-conjugated tetramers identify efficacious anti-HIV CD8+ T-cell specificities | en |
dc.type | Journal Article | en_US |
dc.description.version | Version of Record | en |
dc.relation.journal | PLoS ONE | en |
dash.depositing.author | Leitman, Ellen M. | en_US |
dc.date.available | 2017-12-06T05:55:22Z | |
dc.identifier.doi | 10.1371/journal.pone.0184496 | * |
dash.authorsordered | false | |
dash.contributor.affiliated | Leitman, Ellen | |
dash.contributor.affiliated | Walker, Bruce | |
dc.identifier.orcid | 0000-0001-6122-9245 | |