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dc.contributor.authorWilliams, Charles A. C.en_US
dc.contributor.authorGray, Nathanael S.en_US
dc.contributor.authorFindlay, Greg M.en_US
dc.date.accessioned2017-12-06T05:56:16Z
dc.date.issued2017en_US
dc.identifier.citationWilliams, Charles A. C., Nathanael S. Gray, and Greg M. Findlay. 2017. “A Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screening.” Journal of Visualized Experiments : JoVE (123): 55515. doi:10.3791/55515. http://dx.doi.org/10.3791/55515.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34492443
dc.description.abstractEmbryonic stem cells (ESCs) can self-renew or differentiate into all cell types, a phenomenon known as pluripotency. Distinct pluripotent states have been described, termed "naïve" and "primed" pluripotency. The mechanisms that control naïve-primed transition are poorly understood. In particular, we remain poorly informed about protein kinases that specify naïve and primed pluripotent states, despite increasing availability of high-quality tool compounds to probe kinase function. Here, we describe a scalable platform to perform targeted small molecule screens for kinase regulators of the naïve-primed pluripotent transition in mouse ESCs. This approach utilizes simple cell culture conditions and standard reagents, materials and equipment to uncover and validate kinase inhibitors with hitherto unappreciated effects on pluripotency. We discuss potential applications for this technology, including screening of other small molecule collections such as increasingly sophisticated kinase inhibitors and emerging libraries of epigenetic tool compounds.en
dc.language.isoen_USen
dc.publisherMyJove Corporationen
dc.relation.isversionofdoi:10.3791/55515en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5607952/pdf/en
dash.licenseLAAen_US
dc.subjectDevelopmental Biologyen
dc.subjectIssue 123en
dc.subjectProtein Kinaseen
dc.subjectEmbryonic Stem Cellen
dc.subjectPluripotencyen
dc.subjectNaïve-Primed Transitionen
dc.subjectKinase Inhibitorsen
dc.subjectHigh-Throughput Screenen
dc.titleA Simple Method to Identify Kinases That Regulate Embryonic Stem Cell Pluripotency by High-throughput Inhibitor Screeningen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalJournal of Visualized Experiments : JoVEen
dash.depositing.authorGray, Nathanael S.en_US
dc.date.available2017-12-06T05:56:16Z
dc.identifier.doi10.3791/55515*
dash.contributor.affiliatedGray, Nathanael


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