A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease

DSpace/Manakin Repository

A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease

Citable link to this page

 

 
Title: A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease
Author: Hazelbaker, Dane Z.; Beccard, Amanda; Bara, Anne M.; Dabkowski, Nicole; Messana, Angelica; Mazzucato, Patrizia; Lam, Daisy; Manning, Danielle; Eggan, Kevin; Barrett, Lindy E.

Note: Order does not necessarily reflect citation order of authors.

Citation: Hazelbaker, Dane Z., Amanda Beccard, Anne M. Bara, Nicole Dabkowski, Angelica Messana, Patrizia Mazzucato, Daisy Lam, Danielle Manning, Kevin Eggan, and Lindy E. Barrett. 2017. “A Scaled Framework for CRISPR Editing of Human Pluripotent Stem Cells to Study Psychiatric Disease.” Stem Cell Reports 9 (4): 1315-1327. doi:10.1016/j.stemcr.2017.09.006. http://dx.doi.org/10.1016/j.stemcr.2017.09.006.
Full Text & Related Files:
Abstract: Summary Scaling of CRISPR-Cas9 technology in human pluripotent stem cells (hPSCs) represents an important step for modeling complex disease and developing drug screens in human cells. However, variables affecting the scaling efficiency of gene editing in hPSCs remain poorly understood. Here, we report a standardized CRISPR-Cas9 approach, with robust benchmarking at each step, to successfully target and genotype a set of psychiatric disease-implicated genes in hPSCs and provide a resource of edited hPSC lines for six of these genes. We found that transcriptional state and nucleosome positioning around targeted loci was not correlated with editing efficiency. However, editing frequencies varied between different hPSC lines and correlated with genomic stability, underscoring the need for careful cell line selection and unbiased assessments of genomic integrity. Together, our step-by-step quantification and in-depth analyses provide an experimental roadmap for scaling Cas9-mediated editing in hPSCs to study psychiatric disease, with broader applicability for other polygenic diseases.
Published Version: doi:10.1016/j.stemcr.2017.09.006
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5639480/pdf/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492445
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters