Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01800b Click here for additional data file.
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Hsu, Yen-Pang
Rittichier, Jonathan
Kuru, Erkin
Yablonowski, Jacob
Pasciak, Erick
Tekkam, Srinivas
Hall, Edward
Murphy, Brennan
Lee, Timothy K.
Huang, Kerwyn Casey
Brun, Yves V.
VanNieuwenhze, Michael S.
Note: Order does not necessarily reflect citation order of authors.
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https://doi.org/10.1039/c7sc01800bMetadata
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Hsu, Y., J. Rittichier, E. Kuru, J. Yablonowski, E. Pasciak, S. Tekkam, E. Hall, et al. 2017. “Full color palette of fluorescent d-amino acids for in situ labeling of bacterial cell walls† †Electronic supplementary information (ESI) available. See DOI: 10.1039/c7sc01800b Click here for additional data file.” Chemical Science 8 (9): 6313-6321. doi:10.1039/c7sc01800b. http://dx.doi.org/10.1039/c7sc01800b.Abstract
Fluorescent d-amino acids (FDAAs) enable efficient in situ labeling of peptidoglycan in diverse bacterial species. Conducted by enzymes involved in peptidoglycan biosynthesis, FDAA labeling allows specific probing of cell wall formation/remodeling activity, bacterial growth and cell morphology. Their broad application and high biocompatibility have made FDAAs an important and effective tool for studies of peptidoglycan synthesis and dynamics, which, in turn, has created a demand for the development of new FDAA probes. Here, we report the synthesis of new FDAAs, with emission wavelengths that span the entire visible spectrum. We also provide data to characterize their photochemical and physical properties, and we demonstrate their utility for visualizing peptidoglycan synthesis in Gram-negative and Gram-positive bacterial species. Finally, we show the permeability of FDAAs toward the outer-membrane of Gram-negative organisms, pinpointing the probes available for effective labeling in these species. This improved FDAA toolkit will enable numerous applications for the study of peptidoglycan biosynthesis and dynamics.Other Sources
http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5628581/pdf/Terms of Use
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http://nrs.harvard.edu/urn-3:HUL.InstRepos:34492495
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