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dc.contributor.authorRowlands, Christopher Jen_US
dc.contributor.authorPark, Demianen_US
dc.contributor.authorBruns, Oliver Ten_US
dc.contributor.authorPiatkevich, Kiryl Den_US
dc.contributor.authorFukumura, Daien_US
dc.contributor.authorJain, Rakesh Ken_US
dc.contributor.authorBawendi, Moungi Gen_US
dc.contributor.authorBoyden, Edward Sen_US
dc.contributor.authorSo, Peter TCen_US
dc.date.accessioned2017-12-06T16:18:45Z
dc.date.issued2017en_US
dc.identifier.citationRowlands, Christopher J, Demian Park, Oliver T Bruns, Kiryl D Piatkevich, Dai Fukumura, Rakesh K Jain, Moungi G Bawendi, Edward S Boyden, and Peter TC So. 2017. “Wide-field three-photon excitation in biological samples.” Light, science & applications 6 (1): e16255. doi:10.1038/lsa.2016.255. http://dx.doi.org/10.1038/lsa.2016.255.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34493099
dc.description.abstractThree-photon wide-field depth-resolved excitation is used to overcome some of the limitations in conventional point-scanning two- and three-photon microscopy. Excitation of chromophores as diverse as channelrhodopsins and quantum dots is shown, and a penetration depth of more than 700 μm into fixed scattering brain tissue is achieved, approximately twice as deep as that achieved using two-photon wide-field excitation. Compatibility with live animal experiments is confirmed by imaging the cerebral vasculature of an anesthetized mouse; a complete focal stack was obtained without any evidence of photodamage. As an additional validation of the utility of wide-field three-photon excitation, functional excitation is demonstrated by performing three-photon optogenetic stimulation of cultured mouse hippocampal neurons expressing a channelrhodopsin; action potentials could reliably be excited without causing photodamage.en
dc.language.isoen_USen
dc.relation.isversionofdoi:10.1038/lsa.2016.255en
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5687557/pdf/en
dash.licenseLAAen_US
dc.subjectbiophotonicsen
dc.subjectmultiphoton microscopyen
dc.subjectoptogeneticsen
dc.subjecttemporal focusingen
dc.subjectthree-photonen
dc.titleWide-field three-photon excitation in biological samplesen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalLight, science & applicationsen
dash.depositing.authorFukumura, Daien_US
dc.date.available2017-12-06T16:18:45Z
dc.identifier.doi10.1038/lsa.2016.255*
dash.contributor.affiliatedJain, Rakesh
dash.contributor.affiliatedFukumura, Dai


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