Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters
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CitationBhattacharyya, Sanchari, Shimon Bershtein, and Eugene I Shakhnovich. 2017. “Gene Dosage Experiments in Enterobacteriaceae Using Arabinose-regulated Promoters.” Bio-protocol 7 (14): e2396. doi:10.21769/BioProtoc.2396. http://dx.doi.org/10.21769/BioProtoc.2396.
AbstractThis protocol is used to assay the effect of protein over-expression on fitness of E. coli. It is based on a plasmid expression of a protein of interest from an arabinose-regulated pBAD promoter followed by the measurement of the intracellular protein abundance by Western blot along with the measurement of growth parameters of E. coli cell expressing this protein.
Citable link to this pagehttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34493370
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