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dc.contributor.authorEeckhoute, J.
dc.contributor.authorLupien, M.
dc.contributor.authorMeyer, Clifford
dc.contributor.authorVerzi, M. P.
dc.contributor.authorShivdasani, Ramesh Arjun
dc.contributor.authorLiu, Xiaole (Shirley) Shirley
dc.contributor.authorBrown, Myles A.
dc.date.accessioned2018-01-08T17:32:27Z
dc.date.issued2008
dc.identifier.citationEeckhoute, J., M. Lupien, C. A. Meyer, M. P. Verzi, R. A. Shivdasani, X. S. Liu, and M. Brown. 2008. Cell-Type Selective Chromatin Remodeling Defines the Active Subset of FOXA1-Bound Enhancers. Genome Research 19, no. 3: 372–380. doi:10.1101/gr.084582.108.en_US
dc.identifier.issn1088-9051en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34607114
dc.description.abstractSelective activity of a specific set of enhancers defines tissue-specific gene transcription. The pioneer factor FOXA1 has been shown to induce functional enhancer competency through chromatin openings. We have previously found that FOXA1 is recruited to thousands of regions across the genome of a given cell type. Here, we monitored the chromatin structure at FOXA1 binding sites on a chromosome-wide scale using formaldehyde assisted isolation of regulatory elements (FAIRE). Surprisingly, we find that a significant fraction of FOXA1-bound sites have a relatively closed chromatin conformation linked to a shift of the epigenetic signature toward repressive histone marks. Importantly, these sites are not correlated with gene expression in a given cell type suggesting that FOXA1 is required, but not sufficient, for the functional activity of bound enhancers. Interestingly, we find that a significant proportion of the inactive FOXA1-bound regulatory sites in one cell type are actually functional in another cellular context. We found that at least half of the FOXA1 binding sites from a given cell type are shared with another cell lineage. Mechanisms that restrict the activity of shared FOXA1-bound enhancers likely play a significant role in defining the cell-type-specific functions of FOXA1.en_US
dc.language.isoen_USen_US
dc.publisherCold Spring Harbor Laboratory Pressen_US
dc.relation.isversionofdoi:10.1101/gr.084582.108en_US
dash.licenseLAA
dc.titleCell-type selective chromatin remodeling defines the active subset of FOXA1-bound enhancersen_US
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden_US
dc.relation.journalGenome Researchen_US
dash.depositing.authorLiu, Xiaole (Shirley) Shirley
dc.date.available2018-01-08T17:32:27Z
dc.identifier.doi10.1101/gr.084582.108*
workflow.legacycommentsaa.no Shivdasani emailed for aa 5-6-2017en_US
dash.contributor.affiliatedMeyer, Clifford
dash.contributor.affiliatedBrown, Myles
dash.contributor.affiliatedLiu, Xiaole
dash.contributor.affiliatedShivdasani, Ramesh


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