Characterization of Effector T Cells in Dry Eye Disease

DSpace/Manakin Repository

Characterization of Effector T Cells in Dry Eye Disease

Citable link to this page

 

 
Title: Characterization of Effector T Cells in Dry Eye Disease
Author: Annan, Jaafar; Chauhan, Sunil; Ecoiffier, Tatiana; Zhang, Qiang; Saban, Daniel; Dana, Reza

Note: Order does not necessarily reflect citation order of authors.

Citation: El Annan, Jaafar, Sunil K. Chauhan, Tatiana Ecoiffier, Qiang Zhang, Daniel R. Saban, and Reza Dana. 2009. “Characterization of Effector T Cells in Dry Eye Disease.” Investigative Opthalmology & Visual Science 50 (8) (August 1): 3802. doi:10.1167/iovs.08-2417.
Full Text & Related Files:
Abstract: purpose. Dry eye disease (DED) is associated with ocular surface inflammation that is thought to be mediated primarily by CD4 T cells. The purpose of this study was to investigate whether this T cell–mediated immune response is generated in the lymphoid compartment and to characterize the functional phenotype of the T cells activated in DED.

methods. DED was induced in female C57BL/6 mice by exposure to a desiccating environment in the controlled environment chamber and to systemic scopolamine. T cells from regional draining lymph nodes (LNs) of DED mice and normally sighted mice were analyzed for surface activation markers (CD69 and CD154), chemokine and cytokine receptors, and proliferation potential.

results. Draining LNs of DED mice showed increased frequencies of CD69- and CD154-expressing T cells with higher proliferative capacity. In addition, these LN T cells primarily showed a helper T-cell (Th)1 phenotype, expressing significantly higher levels of IFN-γ and IL-12Rβ2 but not IL-4R. Similarly, the LNs of DED mice showed significantly increased frequencies of T cells expressing CXCR3 and CCR5, but not CCR4, suggesting a bias toward a Th1 phenotype.

conclusions. These data demonstrate that a Th1-type immune response is induced in the regional LNs of DED mice. The identification of specific cytokine/chemokine receptors overexpressed by these T cells may signify potential novel targets/strategies for the treatment of DED.
Published Version: doi:10.1167/iovs.08-2417
Other Sources: http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2921683/
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at http://nrs.harvard.edu/urn-3:HUL.InstRepos:dash.current.terms-of-use#LAA
Citable link to this page: http://nrs.harvard.edu/urn-3:HUL.InstRepos:34622441
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)

 
 

Search DASH


Advanced Search
 
 

Submitters