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dc.contributor.authorShen, Linling
dc.contributor.authorBarabino, Stefano
dc.contributor.authorTaylor, Andrew W.
dc.contributor.authorDana, Reza
dc.date.accessioned2018-01-10T16:37:13Z
dc.date.issued2007
dc.identifierQuick submit: 2017-06-18T19:26:13-0400
dc.identifier.citationShen, Linling. 2007. “Effect of the Ocular Microenvironment in Regulating Corneal Dendritic Cell Maturation.” Archives of Ophthalmology 125 (7) (July 1): 908. doi:10.1001/archopht.125.7.908.en_US
dc.identifier.issn0003-9950en_US
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34622450
dc.description.abstractObjective To determine whether the ocular anterior segment (aqueous humor and cornea) actively inhibits dendritic cell (DC) maturation. Methods Dendritic cells were injected into syngeneic corneas or conjunctivae, and their surface major histocompatibility complex class II expression in response to the local milieu was assessed using confocal microscopy. Immature DCs were cocultured with corneal supernatant or with aqueous humor to evaluate their regulation of DC phenotypic and functional maturity. Results In contrast to conjunctivally injected DCs, DCs injected into the cornea resisted up-regulation in expression of surface major histocompatibility complex class II. Corneal supernatant–treated and aqueous humor–treated DCs retained their immaturity, as reflected by high antigen uptake but low costimulatory molecule (CD80 and CD86) expression and poor T-cell stimulation. Anti–transforming growth factor β2 treatment of aqueous humor and of corneal supernatant led to complete and partial blockade of their inhibition of DC maturation, respectively. However, α-melanocyte–stimulating hormone and calcitonin gene-related peptide had no demonstrable effect on DC maturation. Conclusion Cornea and aqueous humor, principally through transforming growth factor β2, promote generation of phenotypically and functionally immature DCs. Clinical Relevance Our results indicate that relative immune quiescence in the cornea and in the anterior segment is actively maintained in part by the inhibitory effect of transforming growth factor β2 on resident DCs and by their suppression of T-cell–mediated immune and inflammatory responses.en_US
dc.language.isoen_USen_US
dc.publisherAmerican Medical Association (AMA)en_US
dc.relation.isversionof10.1001/archopht.125.7.908en_US
dc.relation.hasversionhttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC4698151/en_US
dash.licenseLAA
dc.titleEffect of the Ocular Microenvironment in Regulating Corneal Dendritic Cell Maturationen_US
dc.typeJournal Articleen_US
dc.date.updated2017-06-18T23:26:15Z
dc.description.versionAccepted Manuscripten_US
dc.relation.journalArchives of Ophthalmologyen_US
dash.depositing.authorDana, Reza
dc.date.available2007
dc.date.available2018-01-10T16:37:13Z
dc.identifier.doi10.1001/archopht.125.7.908*
workflow.legacycommentscat.completeen_US
dash.authorsorderedfalse
dash.contributor.affiliatedDana, Reza


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