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dc.contributor.authorRadaelli, F.en_US
dc.contributor.authorD’Alfonso, L.en_US
dc.contributor.authorCollini, M.en_US
dc.contributor.authorMingozzi, F.en_US
dc.contributor.authorMarongiu, L.en_US
dc.contributor.authorGranucci, F.en_US
dc.contributor.authorZanoni, I.en_US
dc.contributor.authorChirico, G.en_US
dc.contributor.authorSironi, L.en_US
dc.date.accessioned2018-01-18T02:26:21Z
dc.date.issued2017en_US
dc.identifier.citationRadaelli, F., L. D’Alfonso, M. Collini, F. Mingozzi, L. Marongiu, F. Granucci, I. Zanoni, G. Chirico, and L. Sironi. 2017. “μMAPPS: a novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructure.” Scientific Reports 7 (1): 17468. doi:10.1038/s41598-017-17726-y. http://dx.doi.org/10.1038/s41598-017-17726-y.en
dc.identifier.issnen
dc.identifier.urihttp://nrs.harvard.edu/urn-3:HUL.InstRepos:34651825
dc.description.abstractSecond Harmonic Generation (SHG) is a label-free imaging method used to monitor collagen organization in tissues. Due to its sensitivity to the incident polarization, it provides microstructural information otherwise unreachable by other intensity based imaging methods. We develop and test a Microscopic Multiparametric Analysis by Phasor projection of Polarization-dependent SHG (μMAPPS) that maps the features of the collagen architecture in tissues at the micrometer scale. μMAPPS retrieves pixel-by-pixel the collagen fibrils anisotropy and orientation by operating directly on two coupled phasor spaces, avoiding direct fitting of the polarization dependent SHG signal. We apply μMAPPS to fixed tissue sections and to the study of the collagen microscopic organization in tumors ex-vivo and in-vivo. We develop a clustering algorithm to automatically group pixels with similar microstructural features. μMAPPS can perform fast analyses of tissues and opens to future applications for in-situ diagnosis of pathologies and diseases that could assist histo-pathological evaluation.en
dc.language.isoen_USen
dc.publisherNature Publishing Group UKen
dc.relation.isversionofdoi:10.1038/s41598-017-17726-yen
dc.relation.hasversionhttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC5727101/pdf/en
dash.licenseLAAen_US
dc.titleμMAPPS: a novel phasor approach to second harmonic analysis for in vitro-in vivo investigation of collagen microstructureen
dc.typeJournal Articleen_US
dc.description.versionVersion of Recorden
dc.relation.journalScientific Reportsen
dc.date.available2018-01-18T02:26:21Z
dc.identifier.doi10.1038/s41598-017-17726-y*


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