Illuminating Vital Surface Molecules of Symbionts in Health and Disease

DSpace/Manakin Repository

Illuminating Vital Surface Molecules of Symbionts in Health and Disease

Citable link to this page


Title: Illuminating Vital Surface Molecules of Symbionts in Health and Disease
Author: Hudak, Jason E.; Alvarez, David; Skelly, Ashwin; von Andrian, Ulrich H.; Kasper, Dennis L.

Note: Order does not necessarily reflect citation order of authors.

Citation: Hudak, Jason E., David Alvarez, Ashwin Skelly, Ulrich H. von Andrian, and Dennis L. Kasper. 2017. “Illuminating Vital Surface Molecules of Symbionts in Health and Disease.” Nature microbiology 2 (1): 17099. doi:10.1038/nmicrobiol.2017.99.
Full Text & Related Files:
Abstract: The immunomodulatory surface molecules of commensal and pathogenic bacteria are critical to the microbe’s survival and the host’s response.1,2 Recent studies have highlighted the unique and important responses elicited by commensal-derived surface macromolecules;3–5 however, the technology available to track these molecules in host cells and tissues remains primitive. We report here an interdisciplinary approach that uses metabolic labeling combined with bioorthogonal click chemistry (i.e., reactions performed in living organisms)6 to specifically tag up to three prominent surface immunomodulatory macromolecules – peptidoglycan (PGN), lipopolysaccharide (LPS), and capsular polysaccharide (CPS) – either simultaneously or individually in live anaerobic commensal bacteria. Importantly, the PGN labeling enables for the first time the specific labeling of live endogenous, anaerobic bacteria within the mammalian host. This approach has allowed us to image and track the path of labeled surface molecules from live, luminal bacteria into specific intestinal immune cells in the living murine host during health and disease. The chemical labeling of three specific macromolecules within a live organism offers the potential for in-depth visualization of host-pathogen interactions.
Published Version: doi:10.1038/nmicrobiol.2017.99
Other Sources:
Terms of Use: This article is made available under the terms and conditions applicable to Other Posted Material, as set forth at
Citable link to this page:
Downloads of this work:

Show full Dublin Core record

This item appears in the following Collection(s)


Search DASH

Advanced Search